Inflammation-induced modulation of cellular galectin-1 and-3 expression in a model of rat peritonitis

Inflammation-induced modulation of cellular galectin-1 and-3 expression in a model of rat peritonitis

Autor Gil, C. D. Google Scholar
Cooper, D. Google Scholar
Rosignoli, G. Google Scholar
Perretti, M. Google Scholar
Oliani, S. M. Google Scholar
Instituição UNESP
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Queen Mary Sch Med & Dent
Resumo Objective and design: To investigate the effect of galectin-1 (Gal-1) and -3 (Gal-3) on leukocyte migration and analyze the expression of both galectins in inflammatory cells using a model of rat peritonitis.Material or Subjects: Sprague-Dawley rats (n = 4 per group).Treatment: Peritonitis was induced in animals through intraperitoneal injection of carrageenin (1.5 mg/kg) and rat mesenteries were analyzed at different time points (0, 4, 24 and 48h). for pharmacological treatment, rats received intravenous injection of Gal-1 or -3 (3 mu g/kg) followed by carrageenin.Methods: Western blotting and immunoelectron microscopy analysis. Statistical analysis was performed using ANOVA followed by Bonferroni test.Results: Pharmacological treatment with Gal-1, but not Gal-3, inhibited (similar to 50%) leukocyte recruitment into the peritoneal cavity at 4h time-point. in this early phase, immunogold staining of mesenteries showed a diminished Gal-3 expression in degranulated mast cells and Gal-1 in transmigrated neutrophils (similar to 20% reduction compared to intravascular cells). in the later phases (24 and 48 h), leukocyte turnover was associated with augmented Gal-1 expression in neutrophils and macrophages and Gal-3 in mast cells and macrophages.Conclusions: These results point to a balanced expression of cell-associated-Gal-1/Gal-3 and might impact on the development of new therapeutic strategies for inflammatory diseases.
Palavra-chave In vivo inflammation
mast cell
endothelial cell
Idioma Inglês
Data de publicação 2006-03-01
Publicado em Inflammation Research. Basel: Birkhauser Verlag Ag, v. 55, n. 3, p. 99-107, 2006.
ISSN 1023-3830 (Sherpa/Romeo, fator de impacto)
Publicador Birkhauser Verlag Ag
Extensão 99-107
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000236559700003
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