Genetic heterogeneity of Shiga toxin-producing Escherichia coli strains isolated in São Paulo, Brazil, from 1976 through 2003, as revealed by pulsed-field gel electrophoresis

Genetic heterogeneity of Shiga toxin-producing Escherichia coli strains isolated in São Paulo, Brazil, from 1976 through 2003, as revealed by pulsed-field gel electrophoresis

Autor Vaz, Tania Mara I Autor UNIFESP Google Scholar
Irino, Kinue Google Scholar
Nishimura, Lucilia S. Autor UNIFESP Google Scholar
Cergole-Novella, Maria Cecilia Autor UNIFESP Google Scholar
Guth, Beatriz EC Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Adolfo Lutz Registro
Resumo The pulsed-field gel electrophoresis (PFGE) patterns of 46 Shiga toxin-producing Escherichia coli (STEC) strains isolated in São Paulo, Brazil, during the period from 1976 to 2003 were compared with those found among 30 non-STEC strains that carried eae and that belonged to the same serogroups as the STEC strains. All except two of the STEC and non-STEC strains of human origin were from sporadic and unrelated cases of infection; two 0111 strains originated from the same patient. Multiple PFGE patterns were found among STEC strains of distinct serotypes. Moreover, the PFGE restriction patterns of STEC strains differed substantially from those observed among non-STEC strains of the same serogroup except serotype 026 strains. Based on the indistinguishable PFGE pattern for two O157:117 STEC strains isolated in the same geographic area at an interval of approximately 15 days and toxin profile data, the first occurrence of an O157:117 outbreak in Brazil during that period can be suggested. in general, a close relationship between types of intimin, serotypes, and diarrheagenic groups of E. coli was observed. This is the first time that a large collection of STEC strains from Brazil has been analyzed, and a great genetic diversity was shown among O157:117 and non-O157:117 STEC strains isolated in São Paulo, Brazil.
Idioma Inglês
Data de publicação 2006-03-01
Publicado em Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 44, n. 3, p. 798-804, 2006.
ISSN 0095-1137 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 798-804
Fonte http://dx.doi.org/10.1128/JCM.44.3.798-804.2006
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000236095000019
Endereço permanente http://repositorio.unifesp.br/handle/11600/28760

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