Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period

Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period

Autor Horan, Kathleen L. Google Scholar
Freeman, Robert Google Scholar
Weigel, Kris Google Scholar
Semret, Makeda Google Scholar
Pfaller, Stacy Google Scholar
Covert, Terry C. Google Scholar
van Soolingen, Dick Google Scholar
Leao, Sylvia C. Autor UNIFESP Google Scholar
Behr, Marcel A. Google Scholar
Cangelosi, Gerard A. Google Scholar
Instituição Seattle Biomed Res Inst
McGill Univ
Natl Inst Publ Hlth & Environm
Universidade Federal de São Paulo (UNIFESP)
Resumo The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. in the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, these observations also show that strains of the pathogen can be genotypically stable over extended time periods.
Idioma Inglês
Data 2006-03-01
Publicado em Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 44, n. 3, p. 783-789, 2006.
ISSN 0095-1137 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Microbiology
Extensão 783-789
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000236095000017

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