Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains

Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains

Autor Laurino, CCFC Google Scholar
Fritzler, M. J. Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Silva, N. P. Google Scholar
Almeida, I. C. Google Scholar
Andrade, LEC Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Calgary
Univ Texas
Universidade de São Paulo (USP)
Resumo The aim of this study was to characterize a novel human autoantibody-autoantigen system represented as cytoplasmic discrete speckles (CDS) in indirect immunofluorescence (IIF). A distinct CDS IIF pattern represented by 3-20 discrete speckles dispersed throughout the cytoplasm was identified among other cytoplasmic speckled IIF patterns. the cytoplasmic domains labelled by human anti-CDS-1 antibodies did not co-localize with endosome/lysosome markers EEA1 and LAMP-2, but showed partial co-localization with glycine-tryptophan bodies (GWB). CDS-1 sera did not react with several cellular extracts in immunoblotting and did not immunoprecipitate recombinant GW182 or EEA1 proteins. the typical CDS-1 IIF labelling pattern was abolished after delipidation of HEp-2 cells. Moreover, CDS-1 sera reacted strongly with a lipid component co-migrating with phosphatidylethanolamine (PE) in high performance thin-layer chromatography (HPTLC)-immunostaining of HEp-2 cell total lipid extracts. the CDS-1 major molecular targets were established by electrospray ionization-mass spectrometry (ESI-MS), HPTLC-immunostaining and chemiluminescent enzyme-linked immunosorbent assay as diacyl-PE species, containing preferentially a cis-C18 : 1 fatty acid chain at C-2 of the glycerol moiety, namely 1,2-cis-C18 : 1-PE and 1-C16 : 0-2-cis-C18 : 1-PE. the clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease.
Palavra-chave autoantibodies
autoantigen
epitope analysis
cytoplasmic domains
phospholipids
GW bodies
Idioma Inglês
Data de publicação 2006-03-01
Publicado em Clinical and Experimental Immunology. Oxford: Blackwell Publishing, v. 143, n. 3, p. 572-584, 2006.
ISSN 0009-9104 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing
Extensão 572-584
Fonte http://dx.doi.org/10.1111/j.1365-2249.2006.03024.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000235370200022
Endereço permanente http://repositorio.unifesp.br/handle/11600/28755

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