Full-length genome analysis of human immunodeficiency virus type 1 subtype C in Brazil

Full-length genome analysis of human immunodeficiency virus type 1 subtype C in Brazil

Autor Sanabani, S. Google Scholar
Neto, W. K. Google Scholar
De Sa, D. J. Google Scholar
Diaz, R. S. Google Scholar
Munerato, P. Google Scholar
Janini, L. M. Google Scholar
Sabino, E. C. Google Scholar
Instituição Universidade Estadual de Maringá (UEM)
Universidade Federal de São Paulo (UNIFESP)
Resumo The most prevalent HIV-1 clade in the global epidemics is C, and this clade is also becoming important in the Brazilian epidemics. in this study, we characterized HIV-1 subtype C variants by sequencing their near full-length genomes. DNA was extracted from six samples previously classified in our laboratory as subtype C on the basis of partial genome sequencing. Amplification was carried out by overlapping PCR followed by direct sequencing. Phylogenetic analysis of full length genomes confirmed that all isolates belonged to subtype C, which formed a highly supported monophyletic cluster and showed a nucleotide distance of 5.4%. the core promoter of all isolates contained three NF-kappa B binding motifs. Our results suggest that subtype C viruses circulating in Brazil were likely introduced recently from a unique point source. the independent clustering of Brazilian subtype C on the phylogenetic tree suggests the profile of an ideal local candidate for the development of a single subtype vaccine.
Idioma Inglês
Data de publicação 2006-02-01
Publicado em Aids Research and Human Retroviruses. New Rochelle: Mary Ann Liebert Inc, v. 22, n. 2, p. 171-176, 2006.
ISSN 0889-2229 (Sherpa/Romeo, fator de impacto)
Publicador Mary Ann Liebert Inc
Extensão 171-176
Fonte http://dx.doi.org/10.1089/aid.2006.22.171
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000235472200008
Endereço permanente http://repositorio.unifesp.br/handle/11600/28732

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