Glucuronoxylomannan-mediated interaction of Cryptococcus neoformans with human alveolar cells results in fungal internalization and host cell damage

Glucuronoxylomannan-mediated interaction of Cryptococcus neoformans with human alveolar cells results in fungal internalization and host cell damage

Author Barbosa, F. M. Google Scholar
Fonseca, F. L. Google Scholar
Holandino, C. Google Scholar
Alviano, C. S. Google Scholar
Nimrichter, L. Google Scholar
Rodrigues, M. L. Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract Infection by Cryptococcus neoformans begins with inhalation of infectious propagules. Fungi reach the lung tissue and interact with epithelial cells in a crucial but poorly understood process. in this study, the interaction of C. neoformans with the human alveolar epithelial cell lineage A549 was investigated, focusing on the relevance of the capsular polysaccharide in this process. the association of encapsulated strains with A549 cells was significantly inhibited by a monoclonal antibody to glucuronoxylomannan (GXM), a major component of the cryptococcal capsule. A purified preparation of GXM produced similar results, suggesting the occurrence of surface receptors for this polysaccharide on the surface of alveolar cells. A549 cells were in fact able to bind soluble GXM, as confirmed by indirect immunofluorescence analysis using the anti-polysaccharide antibody. C. neoformans is internalized after GXM-mediated interaction with A549 cells in a process that culminates with death of host cells. Our results suggest that C. neoformans can use GXM for attachment to alveolar epithelia, allowing the fungus to reach the intracellular environment and damage host cells through still uncharacterized mechanisms. (c) 2005 Elsevier SAS. All rights reserved.
Keywords glucuronoxylomannan
Cryptococcus neoformans
alveolar epithelium
Language English
Date 2006-02-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 8, n. 2, p. 493-502, 2006.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 493-502
Origin http://dx.doi.org/10.1016/j.micinf.2005.07.027
Access rights Closed access
Type Article
Web of Science ID WOS:000235790200024
URI http://repositorio.unifesp.br/handle/11600/28725

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