CL 316,243, a selective beta(3)-adrenergic agonist, inhibits protein breakdown in rat skeletal muscle

CL 316,243, a selective beta(3)-adrenergic agonist, inhibits protein breakdown in rat skeletal muscle

Autor Navegantes, LCC Google Scholar
Resano, NMZ Google Scholar
Baviera, A. M. Google Scholar
Migliorini, R. H. Google Scholar
Kettelhut, I. C. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Sch Med Sao Jose Rio Preto
Resumo The in vitro effect of CL 316,243 ( CL), a selective beta(3)-adrenoceptor agonist in the rate of overall proteolysis, the activity of proteolytic systems ( lysosomal, Ca2+- dependent, ATP-dependent, and ATP-independent) and in the process of protein synthesis was investigated in rat skeletal muscles. the rate of overall proteolysis in soleus muscle from rats incubated with CL ( 10(-4) and 10(-5) M) or epinephrine (10(-5) M) was significantly decreased. in vitro rates of maximal activity of Ca2+- dependent proteolysis in soleus muscles were decreased by about 41% in the presence of 10(-5) MCL. No change was observed in the activities of the lysosomal, ATP-dependent or ATP-independent proteolytic systems. the anti-proteolytic effect of CL or epinephrine was partially prevented by 10(-5) M SR 59230A, a selective beta(3)-adrenoceptor antagonist. the increase of proteolysis induced by food deprivation in soleus was abolished by in vitro addition of 10(-5) M CL. No change in proteolysis was observed in extensor digitorum longus (EDL) muscles incubated with any concentration of the beta(3)-adrenoceptor agonist tested. Rates of protein synthesis were not affected by 10(-4) M CL neither in soleus nor EDL. the data suggest that a beta(3)-adrenoceptormediated inhibition of Ca2+- dependent proteolysis participates of the antiproteolytic effect of catecholamines in oxidative muscles.
Palavra-chave CL 316,243
beta(3)-agonist
protein degradation
protein synthesis
skeletal muscles
Idioma Inglês
Data de publicação 2006-02-01
Publicado em Pflugers Archiv-european Journal of Physiology. New York: Springer, v. 451, n. 5, p. 617-624, 2006.
ISSN 0031-6768 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 617-624
Fonte http://dx.doi.org/10.1007/s00424-005-1496-1
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234397200003
Endereço permanente http://repositorio.unifesp.br/handle/11600/28720

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