Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats

Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats

Author Andrade, Maria Claudina Camargo de Autor UNIFESP Google Scholar
Di Marco, Giovana Seno Autor UNIFESP Google Scholar
Teixeira, Vicente de Paulo Castro Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Sabatini, Regiane Angelica Autor UNIFESP Google Scholar
Pesquero, Joao Bosco Autor UNIFESP Google Scholar
Boim, Miriam Aparecida Autor UNIFESP Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats. Am J Physiol Renal Physiol 290: F364 - F375, 2006. First published August 16, 2005; doi: 10.1152/ajprenal. 00110.2005. - the angiotensin-converting enzyme (ACE) profile in urine of hypertensive patients and spontaneously hypertensive rats (SHR; 90- and 65-kDa N-domain ACEs) is different from that of healthy subjects and Wistar rats (190 and 65 kDa). in addition, four ACE isoforms were purified from mesangial cells (MC) of Wistar rats in the intracellular compartment (130 and 68 kDa) and as secreted forms (130 and 60 kDa). We decided to characterize ACE forms from SHR MC in culture. Analysis of the ACE gene showed that SHR MC are able to express ACE mRNA. the concentrated medium and cell homogenate were separately purified by gel filtration and then subjected to lisinopril-Sepharose chromatography. the molecular masses of purified enzymes, 90 kDa for ACEm1A and 65 kDa for ACEm2A (secreted enzymes) and 90 kDa for ACEInth1A and 65 kDa for ACEInth2A (intracellular), were different from those of Wistar MC. the purified enzymes are Cl(-) dependent, inhibited by enalaprilat and captopril, and able to hydrolyze AcSDKP. Immunofluorescence and cell fractionation followed by Western blotting showed predominant immunoreaction of the 9B9 antiserum for N-domain ACE in the nuclei. the N-domain ACE was localized in the glomerulus from Wistar rats and SHR. ANG II and ANG-(1-7) were localized in the cell cytoplasm and nuclei. the 90-kDa N-domain ACE, described recently as a possible genetic marker of hypertension, was found inside the cell nuclei of SHR MC colocalized with ANG II and ANG-(1 - 7). the presence of ANG II in the cell nuclei could suggest an important role for this peptide in the transcription of new genes.
Keywords nuclei
hypertension
mesangial cell
regulation of gene expression
Language English
Date 2006-02-01
Published in American Journal of Physiology-renal Physiology. Bethesda: Amer Physiological Soc, v. 290, n. 2, p. F364-F375, 2006.
ISSN 1931-857X (Sherpa/Romeo, impact factor)
Publisher Amer Physiological Soc
Extent F364-F375
Origin http://dx.doi.org/10.1152/ajprenal.00110.2005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000234531200015
URI http://repositorio.unifesp.br/handle/11600/28711

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