Apoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytes

Apoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytes

Autor Hirata, H. Google Scholar
Machado, L. S. Google Scholar
Okuno, C. S. Google Scholar
Brasolin, A. Google Scholar
Lopes, G. S. Google Scholar
Smaili, S. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo In this study, we investigated agents that increased intracellular calcium levels and their correlation with apoptotic cell death induction. We used rat astrocytes to investigate the increase in cytosolic Ca2+, (Ca-c(2+)) and apoptosis induction by drugs that mobilize Ca2+ from different sources. We observed that thapsigargin (Thap), caffeine (Caff) and FCCP which caused similar increases in Ca-c(2+) levels (30-40%), also induced similar apoptotic rates (30-35%). On the other hand, antimycin (Anti), staurosporine (STS) and ethanol (Eth) promoted higher increases in Ca-c(2+) (55-65 %) and higher apoptotic rates (55-85%). Eth induced cell death in a concentration- and time-dependent manner. After treatment with Eth plus Caff for 6, 12 and 24 h, these effects were strongly potentiated. Results suggest that there might be a correlation between Ca-c(2+) increase and the rate of apoptosis. It is possible that Eth induces cell death by activation of more than one pathway and Ca-c(2+) might be one of the elements involved. the present work indicates that Ca-c(2+) can potentiate death by ethanol in rat astrocytes. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Palavra-chave ethanol
calcium
mitochondria
apoptosis
astrocytes
endoplasmic reticulum
Idioma Inglês
Data de publicação 2006-01-30
Publicado em Neuroscience Letters. Clare: Elsevier B.V., v. 393, n. 2-3, p. 136-140, 2006.
ISSN 0304-3940 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 136-140
Fonte http://dx.doi.org/10.1016/j.neulet.2005.09.066
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234583000012
Endereço permanente http://repositorio.unifesp.br/handle/11600/28695

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