Agiong-related changes of intracellular Ca2+ stores and contractile response of intestinal smooth muscle

Agiong-related changes of intracellular Ca2+ stores and contractile response of intestinal smooth muscle

Autor Lopes, G. S. Google Scholar
Ferreira, A. T. Google Scholar
Oshiro, M. E. Google Scholar
Vladmirova, I Google Scholar
Jurkiewicz, N. H. Google Scholar
Jurkiewicz, A. Google Scholar
Smaili, S. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Bogomoletz Inst Phys
Resumo In this study, we investigated the effect of aging on intracellular Ca2+ stores, as sarcoendoplasmic reticulum (SR) and mitochondria, and the influence of these compartments on contraction of rat colon smooth muscle [Bitar, K.N., 2003. Aging and neural control of the GI tract V. Aging and gastrointestinal smooth muscle: from signal transduction to contractile proteins. Am. J. Physiol. Gastrointest. Liver. Physiol. 284(1), G1-G7; Marijic, J., Li, Q.X., Song, M., Nishimaru, K., Stefani, E., Toro, L., 2001. Decreased expression of voltage-and Ca2+-activated K+ channels in coronary smooth muscle during aging. Circ. Res. 88, 210-234; Rubio, C., Moreno, A., Briones, A. Ivorra, M.D., D'Ocon, P., Vila, E., 2002. Alterations by age of calcium handling in rat resistance arteries. J. Cardiovasc. Pharmacol. 40(6), 832-840]. Calcium stores and contraction were evaluated by simultaneous measurements of fluorescence and tension in smooth muscle strips loaded with fura-2. Results showed that activation of muscarinic receptors by methylcholine (MCh, 10 mu M), induced a greater contraction in aged rats than in adult animals. the inhibition of Ca2+ ATPase by thapsigargin (TG, 1 mu M) did not prevent the refilling of SR either in adult or aged rats. MCh, in the presence of TG, induced an increase in transient fluorescence, indicating a release of Ca2+ from TG-insensitive compartment. the mitochondrial uncoupler, FCCP (5 mu M), caused a greater increase in intracellular Ca2+ and tension in aged rats, indicating that mitochondria may accumulate more Ca2+ during aging. the present results show that changes in intracellular Ca2+ stores, such as mitochondria and SR, affect contraction and may cause dysfunctions during aging that could culminate in severe alterations of Ca2+ homeostasis and cell damage.
Palavra-chave calcium store
smooth muscle
Idioma Inglês
Data de publicação 2006-01-01
Publicado em Experimental Gerontology. Oxford: Pergamon-Elsevier B.V., v. 41, n. 1, p. 55-62, 2006.
ISSN 0531-5565 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 55-62
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234788700007
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