Expression of Fas, FasL, and soluble Fas mRNA in endomyocardial biopsies of human cardiac allografts

Expression of Fas, FasL, and soluble Fas mRNA in endomyocardial biopsies of human cardiac allografts

Autor Pérez, Elizabeth Cristina Autor UNIFESP Google Scholar
Shulzhenko, N. Google Scholar
Morgun, A. Google Scholar
Diniz, RVZ Google Scholar
Almeida, D. R. Google Scholar
Musatti, C. C. Google Scholar
Gerbase-DeLima, M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Apoptosis mediated by the Fas/Fas ligand (FasL) has been implicated in rejection of solid organ allografts and it has been recently proposed that soluble forms of Fas could interfere with this interaction, blocking apoptosis. the purpose of this study was to analyze intragraft Fas, FasL, and soluble Fas mRNA levels in relation to acute rejection in cardiac allografts in humans. mRNA levels were determined by quantitative reverse transcriptase-polymerase chain reaction in 42 samples of endomyocardial biopsies obtained from 18 cardiac transplant recipients within the first 6 months after transplantation. FasL and Fas mRNA levels were higher in biopsies with rejection than in biopsies without rejection, and no difference was observed in soluble Fas mRNA. During rejection, there was a positive correlation between the mRNA levels of Fas-FasL, Fas-soluble Fas, and FasL-soluble Fas. During quiescent periods, however, the only correlation observed was between Fas and soluble Fas mRNA levels. in conclusion, our findings do not suggest a role for soluble Fas, confirm the heightened expression of FasL, and indicate, for the first time, an increased expression of Fas in acute rejection of cardiac allografts.
Palavra-chave Fas
Fas ligand
soluble Fas
gene expression
cardiac transplantation
Idioma Inglês
Data de publicação 2006-01-01
Publicado em Human Immunology. New York: Elsevier B.V., v. 67, n. 1-2, p. 22-26, 2006.
ISSN 0198-8859 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 22-26
Fonte http://dx.doi.org/10.1016/j.humimm.2006.02.037
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000237987500003
Endereço permanente http://repositorio.unifesp.br/handle/11600/28644

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