Telomerase activity in prognostic histopathologic features of melanoma

Telomerase activity in prognostic histopathologic features of melanoma

Autor Carvalho, Lucia Google Scholar
Lipay, Monica Google Scholar
Belfort, Francisco Google Scholar
Santos, Ivan Google Scholar
Andrade, Joyce Google Scholar
Haddad, Alessandra Google Scholar
Brunstein, Flavia Google Scholar
Ferreira, Lydia Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Background: Telomerase activity (TA) is believed to play a role in the regulation of senescence and to limit the number of cell divisions. the deregulation of telomerase appears to contribute to oncogenesis and the formation of immortal cell lines. As a result, it is believed that it could be used as a prognostic marker in melanoma.Methods: TA was assayed by the polymerase chain reaction PCR-ELISA-based telomeric repeat amplification protocol (TRAP assay). One hundred and eight samples were distributed in four histological groups: 30 samples from primary cutaneous melanomas, 24 from peritumoural skin sites, 28 from benign melanocytic lesions, and 26 from normal skin sites as a control.Results: TA was different among the four tested groups (Kruskall-Wallis test p < 0.001), and increasing values of TA were observed progressing from normal, skin to benign and then to malignant lesions. Among melanoma samples, there was a significant association between TA and ulceration (p = 0.025), TA and vascular invasion (p = 0.018) and TA and mitotic rate (p = 0.029) (Mann-Whitney test). A linear regression analysis showed significant associations between the increase of TA with Breslow thickness (p = 0.004) and the presence of satellites (p = 0.002).Conclusions: We observed that TA had increased from control skin to peritumoural skin, and then to benign melanocytic lesions and finally to melanoma, suggesting tumour progression. TA showed higher values in the presence of some important histopathologic parameters related to poor prognosis in cutaneous melanoma such as ulceration, vascular invasion, satellites, high rates of mitosis, and in thicker tumours. (C) 2006 the British Association of Plastic Surgeons. Published by Elsevier B.V. All rights reserved.
Assunto telomerase
skin cancer
Idioma Inglês
Data 2006-01-01
Publicado em Journal of Plastic Reconstructive and Aesthetic Surgery. Oxford: Elsevier B.V., v. 59, n. 9, p. 961-968, 2006.
ISSN 1748-6815 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 961-968
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000241204900012

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