Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity

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dc.contributor.author Trindade, M. B.
dc.contributor.author Lopes, JLS
dc.contributor.author Soares-Costa, A.
dc.contributor.author Monteiro-Moreira, A. C.
dc.contributor.author Moreira, R. A.
dc.contributor.author Oliva, MLV
dc.contributor.author Beltramini, L. M.
dc.date.accessioned 2016-01-24T12:38:16Z
dc.date.available 2016-01-24T12:38:16Z
dc.date.issued 2006-01-01
dc.identifier http://dx.doi.org/10.1016/j.bbapap.2005.09.011
dc.identifier.citation Biochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1764, n. 1, p. 146-152, 2006.
dc.identifier.issn 1570-9639
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28640
dc.description.abstract Two novel chitin-binding lectins from seeds of Artocarpus genus were described in this paper, one from A. integrifolia (jackfruit) and one from A. incisa (breadfruit). They were purified from saline crude extract of seeds using affinity chromatography on chitin column, size-exclusion chromatography and reverse-phase chromatography on the C-18 column. Both are 14 kDa proteins, made up of 3 chains linked by disulfide bonds. the partial amino acid sequences of the two lectins showed they are homologous to each other but not to other plant chitin-binding proteins. Thus, they cannot be classified in any known plant chitin-binding protein family, particularly because of their inter-chain covalent bonds. Their circular dichroism spectra and deconvolution showed a secondary structure content of beta-sheet and unordered elements. the lectins were thermally stable until 80 degrees C and structural changes were observed below pH 6. Both lectins inhibited the growth of Fusarium moniliforme and Saccharomyces cerevisiae, and presented hemagglutination activity against human and rabbit erythrocytes. These lectins were denoted jackin (from jackfruit) and frutackin (from breadfruit). (c) 2005 Elsevier B.V. All rights reserved. en
dc.format.extent 146-152
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochimica Et Biophysica Acta-proteins and Proteomics
dc.rights Acesso restrito
dc.subject chitin-binding lectin en
dc.subject lectin from Artocarpus en
dc.subject Fusarium moniliforme en
dc.subject Saccharomyces cerevisiae en
dc.subject antifungal activity en
dc.title Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Carlos (UFSCar)
dc.contributor.institution Univ Fortaleza
dc.contributor.institution Fed Univ Ceara
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Inst Phys, São Paulo, Brazil
dc.description.affiliation Univ Fed Sao Carlos, São Paulo, Brazil
dc.description.affiliation Univ Fortaleza, Fortaleza, Ceara, Brazil
dc.description.affiliation Fed Univ Ceara, Fortaleza, Ceara, Brazil
dc.description.affiliation Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo, Brazil
dc.identifier.doi 10.1016/j.bbapap.2005.09.011
dc.description.source Web of Science
dc.identifier.wos WOS:000235092700018



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