Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity

Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity

Autor Trindade, M. B. Google Scholar
Lopes, JLS Google Scholar
Soares-Costa, A. Google Scholar
Monteiro-Moreira, A. C. Google Scholar
Moreira, R. A. Google Scholar
Oliva, MLV Google Scholar
Beltramini, L. M. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Carlos (UFSCar)
Univ Fortaleza
Fed Univ Ceara
Universidade Federal de São Paulo (UNIFESP)
Resumo Two novel chitin-binding lectins from seeds of Artocarpus genus were described in this paper, one from A. integrifolia (jackfruit) and one from A. incisa (breadfruit). They were purified from saline crude extract of seeds using affinity chromatography on chitin column, size-exclusion chromatography and reverse-phase chromatography on the C-18 column. Both are 14 kDa proteins, made up of 3 chains linked by disulfide bonds. the partial amino acid sequences of the two lectins showed they are homologous to each other but not to other plant chitin-binding proteins. Thus, they cannot be classified in any known plant chitin-binding protein family, particularly because of their inter-chain covalent bonds. Their circular dichroism spectra and deconvolution showed a secondary structure content of beta-sheet and unordered elements. the lectins were thermally stable until 80 degrees C and structural changes were observed below pH 6. Both lectins inhibited the growth of Fusarium moniliforme and Saccharomyces cerevisiae, and presented hemagglutination activity against human and rabbit erythrocytes. These lectins were denoted jackin (from jackfruit) and frutackin (from breadfruit). (c) 2005 Elsevier B.V. All rights reserved.
Palavra-chave chitin-binding lectin
lectin from Artocarpus
Fusarium moniliforme
Saccharomyces cerevisiae
antifungal activity
Idioma Inglês
Data de publicação 2006-01-01
Publicado em Biochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1764, n. 1, p. 146-152, 2006.
ISSN 1570-9639 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 146-152
Fonte http://dx.doi.org/10.1016/j.bbapap.2005.09.011
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000235092700018
Endereço permanente http://repositorio.unifesp.br/handle/11600/28640

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta