Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity

Structural characterization of novel chitin-binding lectins from the genus Artocarpus and their antifungal activity

Author Trindade, M. B. Google Scholar
Lopes, JLS Google Scholar
Soares-Costa, A. Google Scholar
Monteiro-Moreira, A. C. Google Scholar
Moreira, R. A. Google Scholar
Oliva, MLV Google Scholar
Beltramini, L. M. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Carlos (UFSCar)
Univ Fortaleza
Fed Univ Ceara
Universidade Federal de São Paulo (UNIFESP)
Abstract Two novel chitin-binding lectins from seeds of Artocarpus genus were described in this paper, one from A. integrifolia (jackfruit) and one from A. incisa (breadfruit). They were purified from saline crude extract of seeds using affinity chromatography on chitin column, size-exclusion chromatography and reverse-phase chromatography on the C-18 column. Both are 14 kDa proteins, made up of 3 chains linked by disulfide bonds. the partial amino acid sequences of the two lectins showed they are homologous to each other but not to other plant chitin-binding proteins. Thus, they cannot be classified in any known plant chitin-binding protein family, particularly because of their inter-chain covalent bonds. Their circular dichroism spectra and deconvolution showed a secondary structure content of beta-sheet and unordered elements. the lectins were thermally stable until 80 degrees C and structural changes were observed below pH 6. Both lectins inhibited the growth of Fusarium moniliforme and Saccharomyces cerevisiae, and presented hemagglutination activity against human and rabbit erythrocytes. These lectins were denoted jackin (from jackfruit) and frutackin (from breadfruit). (c) 2005 Elsevier B.V. All rights reserved.
Keywords chitin-binding lectin
lectin from Artocarpus
Fusarium moniliforme
Saccharomyces cerevisiae
antifungal activity
Language English
Date 2006-01-01
Published in Biochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1764, n. 1, p. 146-152, 2006.
ISSN 1570-9639 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 146-152
Access rights Closed access
Type Article
Web of Science ID WOS:000235092700018

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