Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

Autor Siu, E. R. Google Scholar
Yasuhara, F. Google Scholar
Marostica, E. Google Scholar
Avellar, MCW Google Scholar
Porto, C. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The expression of muscarinic acetylcholine receptor (mAChR) subtypes (M-1-M-5) was studied in the rat efferent ductules and epididymis at the mRNA and protein levels. the relative abundance of each mAChR transcript subtype differed depending on the tissue and the epididymal region analyzed. the M-1 mAChR mRNA level was more abundant in the efferent ductules than in the caput and cauda of the epididymis. the M-2 mAChR mRNA level was similar between the efferent ductules and caput of the epididymis and higher in the cauda region. the M-3 mAChR mRNA level was low in the efferent ductules and caput of the epididymis, but high levels were detected in the cauda region. mRNAs for M-4 and M-5 mAChRs were not detected in these tissues. Our studies indicated a variable degree of immunostaining for each mAChR subtype in a cell-type and tissue-specific pattern. M-3 mAChR was detected over the efferent ductule epithelium. M-2 and M-3 mAChRs were observed in the apical region of the ciliated cells. Apical and narrow cells of the initial segment showed distinct staining by M-1 antibody, whereas a supranuclear reaction was noted in the principal cells of the caput of the epididymis. in addition, staining for M-1 and M-2 mAChRs was visible in the apical membrane of some epithelial cells of the cauda region. M-3 mAChR was detected in the peritubular smooth muscle of the efferent ductules and epididymis. Functional studies suggested the involvement of this subtype in epididymal tubule contraction. Thus, the cell-specific expression of the various mAChR subtypes in the efferent ductules and epididymis suggests that these receptors play a role in the modulation of luminal fluid composition and smooth muscle contraction.
Palavra-chave epididymis
efferent ductules
muscarinic acetylcholine receptors
rat (wistar)
Idioma Inglês
Data de publicação 2006-01-01
Publicado em Cell and Tissue Research. New York: Springer, v. 323, n. 1, p. 157-166, 2006.
ISSN 0302-766X (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 157-166
Fonte http://dx.doi.org/10.1007/s00441-005-0054-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234427500014
Endereço permanente http://repositorio.unifesp.br/handle/11600/28635

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