Influence of TH1/TH2 switched immune response on renal ischemia-reperfusion injury

Influence of TH1/TH2 switched immune response on renal ischemia-reperfusion injury

Autor Marques, Vilmar Paiva Autor UNIFESP Google Scholar
Gonçalves, Giselle Martins Autor UNIFESP Google Scholar
Feitoza, Carla Quarin Autor UNIFESP Google Scholar
Cenedeze, Marcos Antonio Autor UNIFESP Google Scholar
Bertocchi, Ana Paula Fernandes Autor UNIFESP Google Scholar
Damiao, Marcio Jose Autor UNIFESP Google Scholar
Pinheiro, Helady Sanders Google Scholar
Teixeira, Vicente Paula Antunes Google Scholar
Reis, Marlene Antonia dos Google Scholar
Pacheco-Silva, Alvaro Autor UNIFESP Google Scholar
Camara, Niels Olsen Saraiva Autor UNIFESP Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Background/Aims: Recent evidence shows a critical role of the CD4+ T cell with the Th1/Th2 paradigm as a possible effector mechanism in ischemia and reperfusion injury. We hypothesize that a polarized Th1 activation response may negatively influence the renal IRI through its relationship with chemokine production (MCP-1) and with a protective tissue response (HO-1). Methods: We subjected mice to renal ischemia for 45 min using IL-4 and IL-12 knockout C57BL/6. We then measured serum urea levels, performed histomorphometric analysis for tubular necrosis and regeneration, and evaluated the mRNA expression of HO-1, t-bet, Gata-3 and MCP-1 by real-time PCR at 24,48 and 120 h after surgery. Results/Conclusions: the IL-4 knockout mice had a statistically significant rise in serum urea levels post IRI compared with control animals. the IL-12-deficient mice were not affected. the IL-4-deficient mice had a statistically significant increase in tubular injury and impairment in cell regeneration. the IRI in IL-4-deficient mice was accompanied by higher levels of HO-1, t-bet and later up-regulation of MCP-1. These findings suggest that the deleterious effects of the Th1 cell involve increased production of chemokines such as MCP-1. Copyright (c) 2006 S. Karger AG, Basel.
Assunto ischemia and reperfusion injury
Th1/Th2 cytokines
heme oxygenase 1
Idioma Inglês
Data 2006-01-01
Publicado em Nephron Experimental Nephrology. Basel: Karger, v. 104, n. 1, p. E48-E56, 2006.
ISSN 1660-2129 (Sherpa/Romeo, fator de impacto)
Editor Karger
Extensão E48-E56
Direito de acesso Acesso aberto Open Access
Tipo Resenha
Web of Science WOS:000239896100007

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