Effects of uraemia and dialysis modality on polymorphonuclear cell apoptosis and function

Effects of uraemia and dialysis modality on polymorphonuclear cell apoptosis and function

Autor Sardenberg, Camila Autor UNIFESP Google Scholar
Suassuna, Paulo Autor UNIFESP Google Scholar
Andreoli, Maria Claudia Cruz Autor UNIFESP Google Scholar
Watanabe, Renato Autor UNIFESP Google Scholar
Dalboni, Maria Aparecida Autor UNIFESP Google Scholar
Manfredi, Sílvia Regina Autor UNIFESP Google Scholar
Santos, Oscar Pavão dos Autor UNIFESP Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Draibe, Sérgio Antônio Autor UNIFESP Google Scholar
Cendoroglo Neto, Miguel Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Background. Previous studies have reported that incubation of polymorphonuclear cells (PMN) in uraemic plasma or with different haemodialysis membranes and peritoneal dialysis solutions increases apoptosis in this cell type. in addition, PMN harvested from uraemic patients show a reduced ability to generate superoxide in response to stimuli as well as impaired phagocytosis, chemotaxis and degranulation. the aim of the current study was to investigate the effect of uraemia and dialysis modality on apoptosis and function in freshly harvested non-incubated PMN.Methods. Polymorphonuclear cells were harvested from 14 chronic haemodialysis (HD) patients, from 14 continuous peritoneal dialysis patients (CAPD), 28 chronic kidney disease (CKD), pre-dialysis patients and from 14 healthy subjects (Controls). in these in vivo experiments, PMN apoptosis was studied by means of flow cytometric analysis of annexin V binding to freshly isolated cells. Polymorphonuclear cell phagocytosis and production of reactive oxygen species by unstimulated or stimulated (S.aureus, fMLP, PMA) cells were also studied by flow cytometry using whole blood.Results. We observed increased PMN apoptosis in CKD patients. CAPD and HD patients displayed PMN apoptosis rates similar to controls. in the HD group, PMN exhibited decreased phagocytosis rates. in contrast, phagocytosis rates in PMN from CAPD were not significantly different from controls. in the CKD and HD groups, apoptosis was inversely correlated with respiratory burst activity and phagocytosis.Conclusion. Our results suggest that both uraemia and treatment modality may interfere with PMN apoptosis and function. Dialysis appears to normalize the increased PMN apoptosis rates observed in CKD patients.
Palavra-chave apoptosis
dialysis
immune dysfunction
polymorphonuclear cells
uraemia
Idioma Inglês
Data de publicação 2006-01-01
Publicado em Nephrology Dialysis Transplantation. Oxford: Oxford Univ Press, v. 21, n. 1, p. 160-165, 2006.
ISSN 0931-0509 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press
Extensão 160-165
Fonte http://dx.doi.org/10.1093/ndt/gfi095
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000234436100031
Endereço permanente http://repositorio.unifesp.br/handle/11600/28605

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