Isolation of Leishmania (Viannia) braziliensis glycolipid antigens and their reactivity with mAb SST-1, specific for parasites of Viannia subgenus

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dc.contributor.author Silveira, TGV
dc.contributor.author Yoneyama, KAG
dc.contributor.author Takahashi, H. K.
dc.contributor.author Straus, A. H.
dc.date.accessioned 2016-01-24T12:38:12Z
dc.date.available 2016-01-24T12:38:12Z
dc.date.issued 2005-12-01
dc.identifier http://dx.doi.org/10.1017/S0031182005008498
dc.identifier.citation Parasitology. New York: Cambridge Univ Press, v. 131, p. 737-745, 2005.
dc.identifier.issn 0031-1820
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28586
dc.description.abstract Specific glycolipids (GLs) from Leishmania (Viannia) braziliensis promastigotes were isolated and purified. A monoclonal antibody directed to carbohydrate epitopes of these GLs was produced. mAb SST-1 recognizes a low molecular weight GL as established by solid-phase radioimmunoassay and HPTLC immunostaining, and does not cross-react with lipophosphoglycan isolated from L. (V.) braziliensis promastigotes. An indirect immunofluorescence study indicated that the antigenic GLs are present at the L. (V.) braziliensis promastigote surface. SST-1 reacted with promastigotes of L. (V.) naiffi and L. (V.) guyanensis, but not with species in the L. Leishmania subgenus i.e. L. (L.) amazonensis, L. (L.) chagasi, or L. (L.) major. All L. (V.) braziliensis serodemes tested were reactive with SST-1. These results indicate that SST-1 recognizes specific GLs expressed by species of the Viannia subgenus, and will be particularly useful for identification of L. (V.) braziliensis. en
dc.format.extent 737-745
dc.language.iso eng
dc.publisher Cambridge Univ Press
dc.relation.ispartof Parasitology
dc.rights Acesso restrito
dc.subject Leishmania (Viannia) braziliensis en
dc.subject monoclonal antibody en
dc.subject glycolipid en
dc.subject promastigotes en
dc.title Isolation of Leishmania (Viannia) braziliensis glycolipid antigens and their reactivity with mAb SST-1, specific for parasites of Viannia subgenus en
dc.type Artigo
dc.rights.license http://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Estadual de Maringá (UEM)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, Brazil
dc.description.affiliation Univ Estadual Maringa, Dept Anal Clin, BR-87020900 Maringa, Parana, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1017/S0031182005008498
dc.description.source Web of Science
dc.identifier.wos WOS:000234519600002



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