Isolation of Leishmania (Viannia) braziliensis glycolipid antigens and their reactivity with mAb SST-1, specific for parasites of Viannia subgenus

Isolation of Leishmania (Viannia) braziliensis glycolipid antigens and their reactivity with mAb SST-1, specific for parasites of Viannia subgenus

Autor Silveira, TGV Google Scholar
Yoneyama, KAG Google Scholar
Takahashi, H. K. Google Scholar
Straus, A. H. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Maringá (UEM)
Resumo Specific glycolipids (GLs) from Leishmania (Viannia) braziliensis promastigotes were isolated and purified. A monoclonal antibody directed to carbohydrate epitopes of these GLs was produced. mAb SST-1 recognizes a low molecular weight GL as established by solid-phase radioimmunoassay and HPTLC immunostaining, and does not cross-react with lipophosphoglycan isolated from L. (V.) braziliensis promastigotes. An indirect immunofluorescence study indicated that the antigenic GLs are present at the L. (V.) braziliensis promastigote surface. SST-1 reacted with promastigotes of L. (V.) naiffi and L. (V.) guyanensis, but not with species in the L. Leishmania subgenus i.e. L. (L.) amazonensis, L. (L.) chagasi, or L. (L.) major. All L. (V.) braziliensis serodemes tested were reactive with SST-1. These results indicate that SST-1 recognizes specific GLs expressed by species of the Viannia subgenus, and will be particularly useful for identification of L. (V.) braziliensis.
Palavra-chave Leishmania (Viannia) braziliensis
monoclonal antibody
glycolipid
promastigotes
Idioma Inglês
Data de publicação 2005-12-01
Publicado em Parasitology. New York: Cambridge Univ Press, v. 131, p. 737-745, 2005.
ISSN 0031-1820 (Sherpa/Romeo, fator de impacto)
Publicador Cambridge Univ Press
Extensão 737-745
Fonte http://dx.doi.org/10.1017/S0031182005008498
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234519600002
Endereço permanente http://repositorio.unifesp.br/handle/11600/28586

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