Polymorphisms of the TP53 codon 72 and WRN codon 1367 in individuals from Northern Brazil with gastric adenocarcinoma

Polymorphisms of the TP53 codon 72 and WRN codon 1367 in individuals from Northern Brazil with gastric adenocarcinoma

Autor Khayat, A. S. Google Scholar
Gatti, L. L. Google Scholar
Lima, E. M. Google Scholar
Assumpcao, P. P. de Google Scholar
Motta, FJN Google Scholar
Harada, M. L. Google Scholar
Casartelli, C. Google Scholar
Payao, SLM Google Scholar
Smith, MAC Google Scholar
Burbano, R. R. Google Scholar
Instituição Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Fac Med Marilia
Universidade de São Paulo (USP)
Universidade Federal do Pará (UFPA)
Resumo Gastric cancer is the second most frequent type of neoplasia and also the second most common cause of death in the world. TP53 codon 72, which produces variant proteins with an arginine (Arg) or proline (Pro), has been reported to be associated with cancers of the lung, oesophagus, stomach and cervix. Werner's syndrome (WS) is a premature ageing disease caused by a mutation in the WRN gene. the WRN protein acts as a DNA helicase and as an exonuclease. WRN codon 1367 produces variant proteins with an Arg or cysteine (Cys). This polymorphism has been studied, in order to understand the clinical impact of the molecular variants in WS and in age-related disorders. in the present study, the TP53 codon 72 and the WRN codon 1367 polymorphisms were investigated in 54 gastric adenocarcinoma patients (31 diffuse-type and 25 intestinal-type) and 54 controls. DNA samples were extracted, and PCR-RFLP was utilised for genotyping TP53 codon 72 and WRN codon 1367. the allele frequencies of the TP53 polymorphism were: Arg=0.74 and Pro=0.26. the allele frequencies of the WRN polymorphism were: Cys=0.73 and Arg=0.27. the crude genotypic frequencies in gastric cancer patients were similar to those of the controls, but in the WRN codon 1367 polymorphisms the mean age tended to be higher in the Arg/Arg genotypes. There also was an association, although not statistically significant, between the presence of Helicobacter pylori and the genotypes Cys/Cys and Cys/Arg and a higher percentage of cardia cancer among the Arg/Arg genotypes, and of non-cardia cancer among genotypes Cys/Cys and Cys/Arg. These findings may be a reflection of differences in the interaction between WRN codon 1367 polymorphisms and local factors in the stomach. To our knowledge, this is the first study to examine a genetic polymorphism of the WRN gene in cancer. the precise mechanisms of action of the TP53 and WRN polymorphisms involved in the aetiopathogeny of this disease need further investigation.
Palavra-chave TP53 codon 72 polymorphisms
WRN codon 1367 polymorphisms
gastric cancer
Idioma Inglês
Data de publicação 2005-12-01
Publicado em Clinical and Experimental Medicine. New York: Springer, v. 5, n. 4, p. 161-168, 2005.
ISSN 1591-8890 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 161-168
Fonte http://dx.doi.org/10.1007/s10238-005-0081-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000234053900003
Endereço permanente http://repositorio.unifesp.br/handle/11600/28573

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