Structure, cellular distribution, antigenicity, and biological functions of Fonsecaea pedrosoi ceramide monohexosides

Structure, cellular distribution, antigenicity, and biological functions of Fonsecaea pedrosoi ceramide monohexosides

Autor Nimrichter, Leonardo Autor UNIFESP Google Scholar
Cerqueira, Mariana D. Google Scholar
Leitao, Eduardo A. Google Scholar
Miranda, Kildare Google Scholar
Nakayasu, Ernesto S. Google Scholar
Almeida, Sandro R Google Scholar
Almeida, Igor C. Google Scholar
Alviano, Celuta S. Google Scholar
Barreto-Bergter, Eliana Google Scholar
Rodrigues, Marcio L. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Norte Fluminense Darcy Ribeiro Camp
Universidade de São Paulo (USP)
Univ Texas
Resumo Monohexosylceramides (CMHs, or cerebrosides) have been reported as membrane and cell wall constituents of both pathogenic and nonpathogenic fungi, presenting remarkable differences in their ceramide moiety compared to mammalian CMHs. Current evidence suggests that CMHs are involved in fungal differentiation and growth and contribute to host immune response. Here we describe a structural diversity between cerebrosides obtained from different forms of the human pathogen Fonsecaea pedrosoi. the major CMH species produced by conidial forms displayed the same structure previously demonstrated by our group for mycelia, an N-2'-hydroxyhexadecanoyl-1-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine. However, the major cerebroside species purified from sclerotic cells carries an additional hydroxyl group, bound to its long-chain base. the structural difference between cerebrosides from mycelial and sclerotic cells was apparently not relevant for their antigenicity, since they were both recognized at similar levels by sera from individuals with chromoblastomycosis and a monoclonal antibody to a conserved cerebroside structure. Preincubation of fungal cells with anti-CMH monoclonal antibodies had no effect on the interaction of F. pedrosoi sclerotic cells with murine macrophages. in contrast to what has been described for other fungal species, sclerotic bodies are resistant to the antifungal action of anti-CMH antibodies. Immunofluorescence analysis showed that recognition of sclerotic cells by these antibodies only occurs at cell wall regions in which melanization is not evident. Accordingly, melanin removal with alkali results in an increased reaction of fungal cells with anti-CMH antibodies. Our results indicate that cerebroside expression in F. pedrosoi cells is associated with dimorphism and melanin assembly on the fungal cell wall.
Idioma Inglês
Data 2005-12-01
Publicado em Infection and Immunity. Washington: Amer Soc Microbiology, v. 73, n. 12, p. 7860-7868, 2005.
ISSN 0019-9567 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Microbiology
Extensão 7860-7868
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000233480200009

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