Angiotensin II AT(1) receptor mutants expressed in CHO cells caused morphological change and inhibition of cell growth

Angiotensin II AT(1) receptor mutants expressed in CHO cells caused morphological change and inhibition of cell growth

Autor Corrêa, Silvana Aparecida Alves Autor UNIFESP Google Scholar
Pacheco, NAS Google Scholar
Costa-Neto, C. M. Google Scholar
Oliveira, L. Google Scholar
Pesquero, J. B. Google Scholar
Han, S. W. Google Scholar
Paiva, ACM Google Scholar
Shimuta, S. I. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
São Paulo State Univ
Resumo To assess the importance of the leucine residues in positions 262 and 265 of the angiotensin AT, receptor for signaling pathways and receptor expression and regulation, we compared the properties of CHO cells transfected with the wild type or the L262D or L265D receptor point mutants. It was found that the two mutants significantly increased the basal intracellular cyclic AMP (cAMP) formation in an agonist-independent mode. the morphology transformation of CHO cells was correlated with the increased cAMP formation, since forskolin, a direct activator of adenylate cyclase mimicked this effect on WT-expressing CHO cells. DNA synthesis was found to be inhibited in these cell lines, indicating that cAMP may also have determined the inhibitory effect on cell growth, in addition to the cell transformation from a tumorigenic to a non-tumorigenic phenotype. However a role for an increased Ca2(+) influx induced by the mutants in non-stimulated cells cannot be ruled out since this ion also was shown to cause transformed cells to regain the morphology and growth regulation. (c) 2005 Elsevier B.V. All rights reserved.
Palavra-chave AT(1) receptor
mutagenesis
cyclic AMP
Ca+2 signaling
cell proliferation
morphology regulation
Idioma Inglês
Data de publicação 2005-11-15
Publicado em Regulatory Peptides. Amsterdam: Elsevier B.V., v. 131, n. 1-3, p. 18-22, 2005.
ISSN 0167-0115 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 18-22
Fonte http://dx.doi.org/10.1016/j.regpep.2005.05.005
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000232709100003
Endereço permanente http://repositorio.unifesp.br/handle/11600/28551

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta