Antigen retrieval methods and estrogen receptor immunoexpression using ID5 antibody: A comparative study

Antigen retrieval methods and estrogen receptor immunoexpression using ID5 antibody: A comparative study

Autor Neves, J. I. Google Scholar
Begnami, M. D. Google Scholar
Arias, V Google Scholar
Santos, G. C. Google Scholar
Instituição AC Camargo Hosp
Samaritano Hosp
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Long-term formalin tissue fixation results in antigen masking, probably through aldehydic linkage between proteins and fixative molecules. Immunohistochemistry results depend on the type of the detection procedure and the type of antibody used for the reaction. Considering the difficulty in working with estrogen receptor (ER) antibodies and the lack of standardization of the antigen retrieval methods, we quantified the immunoexpression of ER using the ID5 antibody and a standard streptavidin-biotin detection procedure retrieving with microwave even, steamer, pressure cooker, and water bath in a set of SBR grade 2 invasive breast carcinomas. Pressure-cooking provided the best results. No significant differences were observed in using the other methods. Pressure-cooking should be recommended as the method of choice for standardization of the ER immuohistochemical reaction.
Palavra-chave breast carcinoma
immunohistochemistry
prognostic factors
predictive factors
antigen retrieval methods
Idioma Inglês
Data de publicação 2005-10-01
Publicado em International Journal of Surgical Pathology. Glen Head: Westminster Publ Inc, v. 13, n. 4, p. 353-357, 2005.
ISSN 1066-8969 (Sherpa/Romeo, fator de impacto)
Publicador Westminster Publ Inc
Extensão 353-357
Fonte http://dx.doi.org/10.1177/106689690501300407
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000233122000007
Endereço permanente http://repositorio.unifesp.br/handle/11600/28504

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