Apoptosis in the epithelial cells of the rests of Malassez of the periodontium of rat molars

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dc.contributor.author Cerri, P. S.
dc.contributor.author Katchburian, E.
dc.date.accessioned 2016-01-24T12:38:06Z
dc.date.available 2016-01-24T12:38:06Z
dc.date.issued 2005-10-01
dc.identifier http://dx.doi.org/10.1111/j.1600-0765.2005.00810.x
dc.identifier.citation Journal of Periodontal Research. Oxford: Blackwell Publishing, v. 40, n. 5, p. 365-372, 2005.
dc.identifier.issn 0022-3484
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28503
dc.description.abstract Background and Objectives: Epithelial rests of Malassez are clusters of cells derived from Hertwig's root sheath that remain in the periodontal ligament throughout life. Although it is known that the cells of Malassez proliferate, there are no studies showing that they undergo programmed cell death, i.e. apoptosis. in most tissues, proliferation is balanced by apoptosis. Thus we examined regions of the periodontium of young and adult rat molars in the hope of detecting apoptosis.Methods: Wistar rats aged 29, 45 and 120 days were killed with chloral hydrate (600 mg/kg). Fragments containing maxillary molars were removed and fixed in formaldehyde, decalcified, and embedded in paraffin and glycol methacrylate. Sections were stained with hematoxylin/eosin and the Terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labeling (TUNEL) method for detection of apoptosis. Specimens were also fixed in glutaraldehyde-formaldehyde, decalcified and processed for transmission electron microscopy.Results: Epithelial rests of Malassez containing round/ovoid basophilic dense bodies and TUNEL-positive structures were found in all specimens examined. Ultrastructural examination revealed that some cells of Malassez contained masses of condensed peripheral chromatin and a shrunken cytoplasm exhibiting intact organelles - images typical of apoptosis. Moreover, round/ovoid electron-opaque structures appeared to be in the process of being engulfed by neighboring epithelial cells of Malassez.Conclusions: Our results demonstrate that epithelial cells of Malassez's rests undergo apoptosis in the developing and adult periodontium. Apoptosis may, together with proliferation, be part of the mechanism of turnover/remodelling of the cells of Malassez. en
dc.format.extent 365-372
dc.language.iso eng
dc.publisher Blackwell Publishing
dc.relation.ispartof Journal of Periodontal Research
dc.rights Acesso restrito
dc.subject apoptosis en
dc.subject epithelial cells en
dc.subject periodontium en
dc.subject rests of Malassez en
dc.title Apoptosis in the epithelial cells of the rests of Malassez of the periodontium of rat molars en
dc.type Artigo
dc.contributor.institution UNESP
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNESP, Dept Morphol, Sch Dent, Dept Morphol, BR-14801903 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, EPM, Dept Morphol, Sch Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, EPM, Dept Morphol, Sch Med, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1600-0765.2005.00810.x
dc.description.source Web of Science
dc.identifier.wos WOS:000231145900001

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