Disruption of myofibrillar proteins in cardiac muscle of Calomys callosus chronically infected with Trypanosoma cruzi and treated with immunosuppressive agent

Disruption of myofibrillar proteins in cardiac muscle of Calomys callosus chronically infected with Trypanosoma cruzi and treated with immunosuppressive agent

Autor Taniwaki, N. N. Google Scholar
Andreoli, W. K. Google Scholar
Calabrese, K. S. Google Scholar
Silva, S. da Google Scholar
Mortara, R. A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Adolfo Lutz Registro
Dept Protozool
Resumo Calomys callosus (Rodentia: Cricetidae) chronically infected with CL strain of Trypanosoma cruzi undergo recrudescence of the acute phase when treated with the immunosuppressor cyclophosphamide. the distribution of cytoskeletal proteins in cardiac tissue of immunosuppressed animals was mapped by immunofluorescence and electron microscopy to evaluate myofibrillar distribution during the intracellular life cycle of T. cruzi. Cardiac muscle sections showed enhancement of myocarditis and parasite proliferation after immunosuppression. Immunofluorescence using monoclonal antibodies against myosin, actin, desmin, titin, tropomyosin, and troponin T demonstrated disruption and loss of contractile proteins, such as myosin and actin. Desmin and titin were irregularly distributed in close proximity to parasite nests. Ultrastructural observations confirmed alterations of cardiac cells with Z-line fragmentation, indistinguishable I-bands and A-bands, and loss of myofibrillar elements. the disruption of the muscle cell architecture was greater as infection progressed, probably as a result of increased myocarditis and physical displacement due to the activity of flagellated parasites.
Palavra-chave Trypanosoma cruz
Calomys callosus
cardiac myofibrillar proteins
confocal microscopy
electron microscopy
Idioma Inglês
Data de publicação 2005-10-01
Publicado em Parasitology Research. New York: Springer, v. 97, n. 4, p. 323-331, 2005.
ISSN 0932-0113 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 323-331
Fonte http://dx.doi.org/10.1007/s00436-005-1429-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000232662700011
Endereço permanente http://repositorio.unifesp.br/handle/11600/28486

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta