Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America

Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America

Author Ponce, Carlos Google Scholar
Ponce, Elisa Google Scholar
Vinelli, Elizabeth Google Scholar
Montoya, Alberto Google Scholar
Aguilar, Vilma de Google Scholar
Gonzalez, Antonio Google Scholar
Zingales, Bianca Google Scholar
Rangel-Aldao, Rafael Google Scholar
Levin, Mariano J. Google Scholar
Esfandiari, Javan Google Scholar
Umezawa, Eufrosina S. Google Scholar
Luquetti, Alejandro O. Google Scholar
Silveira, Jose Franco da Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Secretaria Salud
Cruz Roja Hondurena
Minist Salud
Minist Salud Publ & Asistencia Social
CSIC
Universidade de São Paulo (USP)
Univ Simon Bolivar
Inst Invest Engn Genet & Biol Mol
Chembio Diagnost Syst Inc
Universidade Federal de Goiás (UFG)
Abstract In this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas' disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. in conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America.
Language English
Date 2005-10-01
Published in Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 10, p. 5065-5068, 2005.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 5065-5068
Origin http://dx.doi.org/10.1128/JCM.43.10.5065-5068.2005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000232762500020
URI http://repositorio.unifesp.br/handle/11600/28480

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