Adjuvant effect of synthetic oligodeoxyribonucleotides (CpG-ODN) from the Paracoccidioides brasiliensis gp43 gene on the Th2-Th1 immunomodulation of experimental paracoccidioidomycosis

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dc.contributor.author Amaral, C. C. [UNIFESP]
dc.contributor.author Garcia, I. P. [UNIFESP]
dc.contributor.author Fernandes, Geisa Ferreira [UNIFESP]
dc.contributor.author Almeida, SR
dc.contributor.author Camargo, Zoilo Pires de [UNIFESP]
dc.contributor.author Souza, M. C.
dc.date.accessioned 2016-01-24T12:38:04Z
dc.date.available 2016-01-24T12:38:04Z
dc.date.issued 2005-10-01
dc.identifier http://dx.doi.org/10.1111/j.1365-3083.2005.01680.x
dc.identifier.citation Scandinavian Journal of Immunology. Oxford: Blackwell Publishing, v. 62, n. 4, p. 325-333, 2005.
dc.identifier.issn 0300-9475
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28478
dc.description.abstract Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis. Immunostimulatory effects of P. brasiliensis DNA and CpG-oligodeoxyribonucleotides (CpG-ODN) have shown a Th2-Th1 immunomodulation of the isogenic murine model of susceptibility, which develops a progressive and disseminating disease. in this study, we investigated the optimum time interval and doses of CpG-ODN which are able to induce Th2-Th1 immunomodulation. the optimum concentrations for the induction of a decrease in antibody production were 0.5 and 1 mu g. Mice immunized twice with CpG-ODN and gp43 (5 and 7 days before the challenge) showed a 60% higher chance of survival compared with the control group (nonimmunized), and an increase in Th1 isotype (IgG2a) was also observed. in vitro assays of naive and preimmunized mice showed discrete cellular proliferation when stimulated by suitable concentrations of CpG-ODN. Type 1 cytokines interleukin-12 (IL-12) and interferon-gamma were increased in cell culture supernatants, but no significant difference was found in Th2 IL-4 cytokines in stimulated or nonstimulated cell cultures. Concerning the Th2-Th1 kinetics in experimental PCM models by adjuvant effect of CpG-ODN, there are still many questions to be answered and clarified. However, the gathering of data obtained in this investigation has led us to suggest that the modulation of Th2-Th1 in experimental PCM depends on time and CpG-ODN concentration. en
dc.format.extent 325-333
dc.language.iso eng
dc.publisher Blackwell Publishing
dc.relation.ispartof Scandinavian Journal of Immunology
dc.rights Acesso aberto
dc.title Adjuvant effect of synthetic oligodeoxyribonucleotides (CpG-ODN) from the Paracoccidioides brasiliensis gp43 gene on the Th2-Th1 immunomodulation of experimental paracoccidioidomycosis en
dc.type Artigo
dc.contributor.institution UMESP
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation UMESP, BR-09895400 Sao Bernardo Do Campo, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Clin & Toxicol Anal, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1365-3083.2005.01680.x
dc.description.source Web of Science
dc.identifier.wos WOS:000232774600001



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