Adjuvant effect of synthetic oligodeoxyribonucleotides (CpG-ODN) from the Paracoccidioides brasiliensis gp43 gene on the Th2-Th1 immunomodulation of experimental paracoccidioidomycosis

Adjuvant effect of synthetic oligodeoxyribonucleotides (CpG-ODN) from the Paracoccidioides brasiliensis gp43 gene on the Th2-Th1 immunomodulation of experimental paracoccidioidomycosis

Author Amaral, C. C. Autor UNIFESP Google Scholar
Garcia, I. P. Autor UNIFESP Google Scholar
Fernandes, Geisa Ferreira Autor UNIFESP Google Scholar
Almeida, SR Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Souza, M. C. Google Scholar
Institution UMESP
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis. Immunostimulatory effects of P. brasiliensis DNA and CpG-oligodeoxyribonucleotides (CpG-ODN) have shown a Th2-Th1 immunomodulation of the isogenic murine model of susceptibility, which develops a progressive and disseminating disease. in this study, we investigated the optimum time interval and doses of CpG-ODN which are able to induce Th2-Th1 immunomodulation. the optimum concentrations for the induction of a decrease in antibody production were 0.5 and 1 mu g. Mice immunized twice with CpG-ODN and gp43 (5 and 7 days before the challenge) showed a 60% higher chance of survival compared with the control group (nonimmunized), and an increase in Th1 isotype (IgG2a) was also observed. in vitro assays of naive and preimmunized mice showed discrete cellular proliferation when stimulated by suitable concentrations of CpG-ODN. Type 1 cytokines interleukin-12 (IL-12) and interferon-gamma were increased in cell culture supernatants, but no significant difference was found in Th2 IL-4 cytokines in stimulated or nonstimulated cell cultures. Concerning the Th2-Th1 kinetics in experimental PCM models by adjuvant effect of CpG-ODN, there are still many questions to be answered and clarified. However, the gathering of data obtained in this investigation has led us to suggest that the modulation of Th2-Th1 in experimental PCM depends on time and CpG-ODN concentration.
Language English
Date 2005-10-01
Published in Scandinavian Journal of Immunology. Oxford: Blackwell Publishing, v. 62, n. 4, p. 325-333, 2005.
ISSN 0300-9475 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 325-333
Origin http://dx.doi.org/10.1111/j.1365-3083.2005.01680.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000232774600001
URI http://repositorio.unifesp.br/handle/11600/28478

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