Developing skeletal muscle cells express functional muscarinic acetylcholine receptors coupled to different intracellular signaling systems

Developing skeletal muscle cells express functional muscarinic acetylcholine receptors coupled to different intracellular signaling systems

Autor Furlan, Ingrid Google Scholar
Godinho, Rosely Oliveira Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo 1 This study analyzed the expression of muscarinic acetylcholine receptors (mAChRs) in the rat cultured skeletal muscle cells and their coupling to G protein, phospholipase C and adenylyl cyclase (AC).2 Our results showed the presence of a homogeneous population of [H-3]methyl-quinuclidinyl benzilate-binding sites in the membrane fraction from the rat cultured muscle (K-D = 0.4 nM, B-max = 8.9 fmol mg protein(-1)). Specific muscarinic binding sites were also detected in denervated diaphragm muscles from adult rats and in myoblasts isolated from newborn rats.3 Activation of mAChRs with carbachol induced specific [S-35]GTP gamma S binding to cultured muscle membranes and potentiated the forskolin-dependent stimulation of AC. These effects were totally inhibited by 0.1-1 mu M atropine.4 in addition, mAChRs were able to stimulate generation of diacylglycerol (DAG) in response to acetylcholine, carbachol or selective mAChR agonist oxotremorine-M.5 the carbachol-dependent increase in DAG was inhibited in a concentration-dependent manner by mAChR antagonists atropine, pirenzepine and 4-DAMP mustard.6 Finally, activation of these receptors was correlated with increased synthesis of acetylcholinesterase, via a PKC-dependent pathway.7 Taken together, these results indicate that expression of mAChRs, coupled to G protein and distinct intracellular signaling systems, is a characteristic of noninnervated skeletal muscle cells and may be responsible for trophic influences of acetylcholine during formation of the neuromuscular synapse.
Palavra-chave muscarinic acetylcholine receptors
diacylglycerol
cyclic AMP
skeletal muscle
G protein
development
Idioma Inglês
Data de publicação 2005-10-01
Publicado em British Journal of Pharmacology. London: Nature Publishing Group, v. 146, n. 3, p. 389-396, 2005.
ISSN 0007-1188 (Sherpa/Romeo, fator de impacto)
Publicador Nature Publishing Group
Extensão 389-396
Fonte http://dx.doi.org/10.1038/sj.bjp.0706329
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000232317400010
Endereço permanente http://repositorio.unifesp.br/handle/11600/28475

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