Characterization of RAB-like4, the first identified RAB-like protein from Trypanosoma cruzi with GTPase activity

Characterization of RAB-like4, the first identified RAB-like protein from Trypanosoma cruzi with GTPase activity

Autor Ramos, F. P. Google Scholar
Araripe, JR Google Scholar
Urmenyi, T. P. Google Scholar
Silva, R. Google Scholar
Silva, NLCE Google Scholar
Fontes, CFL Google Scholar
Silveira, J. F. da Google Scholar
Rondinelli, E. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo RAB proteins, which belong to the RAS superfamily, regulate exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion. Few RAB proteins have been identified in parasites. Molecular markers for cellular compartments are important to studies concerning about the protein traffic in Trypanosoma cruzi, the causal agent of Chagas disease. in this work, we describe the characterization of TcRABL4, the first RAB-like gene identified in T. cruzi (GenBank Accession No.: AY371275), present as a single-copy gene. TcRABL4 contains all five consensus RAB motifs but lacks cysteine residues at the C terminus, which are essential to isoprenylation, an absolute prerequisite for membrane association of these proteins. TcRABL4 is a functional GTPase that is able to bind and hydrolyze GTP, and its gene is transcribed as a single 1.2 kb mRNA in epimastigotes. TcRABL4 appears to be differentially regulated in the three cell forms of the parasite, and the protein is not associated to membranes, unlike other RAB proteins. It is possible that TcRABL4 may be a member of a novel family of small GTPases. (c) 2005 Elsevier Inc. All rights reserved.
Palavra-chave GTPase
RAS superfamily
endocytic pathway
Idioma Inglês
Data de publicação 2005-08-05
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 333, n. 3, p. 808-817, 2005.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 808-817
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000230418800021
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