Pseudomonas aeruginosa clonal dissemination in Brazilian intensive care units

Pseudomonas aeruginosa clonal dissemination in Brazilian intensive care units

Autor Figueiredo-Mendes, Caio Marcio Google Scholar
Sinto, Sumiko Google Scholar
Mello-Sampaio, Jorge Luiz Google Scholar
Cardoso-Leao, Sylvia Autor UNIFESP Google Scholar
Oplustil, Carmen Paz Google Scholar
Turner, Philip Google Scholar
Veiga-Kiffer, Carlos Roberto Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
AstraZeneca Pharmaceut
Resumo OBJECTIVE. Investigate clonal dissemination of nosocomial multidrug-resistant Pseudomonas aeruginosa isolates within and between Brazilian intensive care units, which participated in the MYSTIC Program Brazil 2002.METHODS. Thirty-six P. aeruginosa isolates resistant to meropenem or imipenem plus at least two of the following drugs: ciprofloxacin, cefepime, ceftazidime or piperacillin/tazobactam were isolated during 2002 at 4 centres in São Paulo and 1 centre in Brasilia. Chromosomal restriction fragments obtained with Spel were separated by pulsed-field gel electrophoresis (PFGE). Electrophoretic patterns were analyzed with GeICompar II v. 2.5.RESULTS. Five major clones were identified (A, B, C, D, G). Clone A was constituted by 8 isolates with indistinguishable PFGE pattern present in 2 centres. Clone B was constituted by 4 indistinguishable isolates predominant in centre 6. Clone C had 3 indistinguishable isolates, with closely related clones (C1-3). Also, Clone D had 3 indistinguishable isolates, with closely related (D1) and possibly related (D2/D3) clones. Clones C and D were present in centre 1. Clone G was constituted by 2 indistinguishable isolates and was present in centre 7. Finally, 8 isolates were unique. Isolates from Centre 4 were unique.CONCLUSIONS. Clonal dissemination was detected within (clones A, B, C, D, and G) and between centres (clone A). These findings are important when analyzing surveillance data, since susceptibility rates may be significantly affected by the dissemination of a resistant clone.
Palavra-chave pulsed-field gel electrophoresis
Pseudomonas aeruginosa
multi-drug resistance
Idioma Inglês
Data de publicação 2005-08-01
Publicado em Enfermedades Infecciosas Y Microbiologia Clinica. Barcelona: Ediciones Doyma S/l, v. 23, n. 7, p. 402-405, 2005.
ISSN 0213-005X (Sherpa/Romeo, fator de impacto)
Publicador Ediciones Doyma S/l
Extensão 402-405
Fonte http://www.elsevier.es/es-revista-enfermedades-infecciosas-microbiologia-clinica-28-articulo-ipseudomonas-aeruginosa-i-clonal-dissemination-in-13078798
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000231657000003
Endereço permanente http://repositorio.unifesp.br/handle/11600/28395

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