Effect of dietary supplementation with vitamin E and stocking density on macrophage recruitment and giant cell formation in the teleost fish, Piaractus mesopotamicus

Effect of dietary supplementation with vitamin E and stocking density on macrophage recruitment and giant cell formation in the teleost fish, Piaractus mesopotamicus

Author Belo, MAA Google Scholar
Schalch, SHC Google Scholar
Moraes, F. R. Google Scholar
Soares, V. E. Google Scholar
Otoboni, AMMB Google Scholar
Moraes, JER Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The effect of dietary supplementation with 0, 100 and 450 mg of vitamin E (DL-alpha tocopheryl acetate)/kg of a dry diet on the kinetics of macrophage recruitment and giant cell formation in the pacu, maintained at different stocking densities (5 kg/m(3) and 20 kg/m(3)), was investigated by insertion of round glass coverslips into the subcutaneous connective tissue. After a feeding period of 18 weeks, the coverslips were implanted and later removed for examination at 2, 7 and 15 days post-implantation. Fish fed diets supplemented with 450 mg of vitamin E showed an increase (P < 0.05) in the accumulation of macrophages, foreign body giant cells and Langhans type cells. the kinetics of macrophage recruitment and giant cell formation on the glass coverslips appeared to be strongly influenced by vitamin E supplementation, since fish fed a basal diet and held at high stocking densities showed low numbers of adhering cells on the coverslips, and high concentrations of plasma corticosteroids. On the other hand, fish given a diet supplemented with 450 mg of vitamin E did not show a similar difference in plasma cortisol concentrations related to stocking density. the effect of cortisol concentrations on carbohydrate metabolism, analysed by assessment of plasma glycaemia, was not clear. Blood glucose concentrations did not vary substantially with the different treatments examined. These results suggest that vitamin E may contribute to the efficiency of the fish's inflammatory response by increasing macrophage recruitment and giant cell formation in the foreign body granulomatous reaction. Vitamin E appeared to act on the stress response of pacus by preventing a stress-related immunosuppression. (c) 2005 Elsevier B.V. All rights reserved.
Keywords alpha-tocopherol
cortisol
fish
Piaractus mesopotamicus
polykaryon macrophage
vitamin E
Language English
Date 2005-08-01
Published in Journal of Comparative Pathology. London: Academic Press Ltd Elsevier B.V., v. 133, n. 2-3, p. 146-154, 2005.
ISSN 0021-9975 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 146-154
Origin http://dx.doi.org/10.1016/j.jcpa.2005.04.004
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000231570300007
URI http://repositorio.unifesp.br/handle/11600/28387

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