Fucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrix

Fucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrix

Autor Rocha, HAO Google Scholar
Franco, CRC Google Scholar
Trindade, E. S. Google Scholar
Veiga, S. S. Google Scholar
Leite, E. L. Google Scholar
Nader, H. B. Google Scholar
Dietrich, C. P. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Univ Fed Parana
Resumo In recent years, sulfated fucans have emerged as an important class of natural biopolymers. in this study, the anti-adhesive activity of a fucan from the brown seaweed Spatoglossum schroederi was analyzed using tumorigenic cells: wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). Fibronectin (FN) was used as substrate for cell attachment. for both cell types, this fucan has shown a dose-dependent anti-adhesive effect, reaching saturation at around 400 mu g/mL. This effect was abolished by desulfation of the fucan. in addition, this polymer exhibited the highest inhibitory effect in comparison to other sulfated polysaccharides. the fucan was biotinylated and used as a probe to identify its action sites. Biotinylated fucan was detected in the extracellular matrix environment by confocal microscopy and flow cytometric analysis, but not at the cell surface. the results suggest that the fucan shows anti-adhesive activity by binding directly to FN, and blocking FN sites that are recognized by cell surface ligands, possibly the integrin family.
Assunto CHO cells
cell adhesion
anti-adhesive fucan
Spatoglossum schroederi
Idioma Inglês
Data 2005-07-01
Publicado em Planta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 71, n. 7, p. 628-633, 2005.
ISSN 0032-0943 (Sherpa/Romeo, fator de impacto)
Editor Georg Thieme Verlag Kg
Extensão 628-633
Fonte http://dx.doi.org/10.1055/s-2005-871268
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000231031600008
URI http://repositorio.unifesp.br/handle/11600/28377

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