Loss of histochernical identity in mast cells lacking carboxypeptidase A

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dc.contributor.author Feyerabend, Thorsten B.
dc.contributor.author Hausser, Heinz
dc.contributor.author Tietz, Annette
dc.contributor.author Blum, Carmen
dc.contributor.author Hellman, Lars
dc.contributor.author Straus, Anita H. [UNIFESP]
dc.contributor.author Takahashi, Helio K. [UNIFESP]
dc.contributor.author Morgan, Ellen S.
dc.contributor.author Dvorak, Ann M.
dc.contributor.author Fehling, Hans Jorg
dc.contributor.author Rodewald, Hans Reimer
dc.date.accessioned 2016-01-24T12:37:56Z
dc.date.available 2016-01-24T12:37:56Z
dc.date.issued 2005-07-01
dc.identifier http://dx.doi.org/10.1128/MCB.25.14.6199-6210.2005
dc.identifier.citation Molecular and Cellular Biology. Washington: Amer Soc Microbiology, v. 25, n. 14, p. 6199-6210, 2005.
dc.identifier.issn 0270-7306
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28358
dc.description.abstract Mast cell carboxypeptidase A (Mc-cpa) is a highly conserved secretory granule protease. the onset of expression in mast cell progenitors and lineage specificity suggest an important role for Mc-cpa in mast cells. To address the function of Mc-cpa, we generated Mc-cpa-null mice. Mc-cpa(-/-) mast cells lacked carboxypeptidase activity, revealing that Mc-cpa is a nonredundant enzyme. While Mc-cpa(-/-) peritoneal mast cells were ultrastructurally normal and synthesized normal amounts of heparin, they displayed striking histochemical and biochemical hallmarks of immature mast cells. Wild-type peritoneal mast cells had a mature phenotype characterized by differential histochemical staining with proteoglycan-reactive dyes (cells do not stain with alcian blue but stain with safranin and with berberine) and a high side scatter to forward scatter ratio by flow cytometry and were detergent resistant. in contrast, Me-cpa(-/-) peritoneal mast cells, like immature bone marrow-derived cultured mast cells, stained with alcian blue normally or weakly and either did not stain with safranin and berberine or stained weakly, had a low side scatter to forward scatter ratio, and were detergent sensitive. This phenotype was partially ameliorated with age. Thus, histochemistry and flow cytometry, commonly used to measure mast cell maturation, deviated from morphology in Mc-cpa(-/-) mice. the Mc-cpa(-/-) mast cell phenotype was not associated with defects in degranulation in vitro or passive cutaneous anaphylaxis in vivo. Collectively, Mc-cpa plays a crucial role for the generation of phenotypically mature mast cells. en
dc.format.extent 6199-6210
dc.language.iso eng
dc.publisher Amer Soc Microbiology
dc.relation.ispartof Molecular and Cellular Biology
dc.rights Acesso aberto
dc.title Loss of histochernical identity in mast cells lacking carboxypeptidase A en
dc.type Artigo
dc.contributor.institution Univ Ulm
dc.contributor.institution Uppsala Univ
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Beth Israel Deaconess Med Ctr
dc.description.affiliation Univ Ulm, Dept Immunol, D-89070 Ulm, Germany
dc.description.affiliation Univ Ulm, Dept Orthoped, D-89070 Ulm, Germany
dc.description.affiliation Uppsala Univ, Ctr Biomed, Dept Cell & Mol Biol, S-75124 Uppsala, Sweden
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, Brazil
dc.description.affiliation Beth Israel Deaconess Med Ctr, Dept Pathol, Boston, MA 02215 USA
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, Brazil
dc.identifier.file WOS000230267000033.pdf
dc.identifier.doi 10.1128/MCB.25.14.6199-6210.2005
dc.description.source Web of Science
dc.identifier.wos WOS:000230267000033

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