Characterization of an ecto-ATPase activity in Cryptococcus neoformans

Characterization of an ecto-ATPase activity in Cryptococcus neoformans

Autor Junior, I. C. Google Scholar
Rodrigues, M. L. Google Scholar
Alviano, C. S. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Meyer-Fernandes, JR Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo Cryptococcus neoformans is the causative agent of pulmonary cryptococcosis and cryptococcal meningoencephalitis, which are major clinical manifestations in immunosuppressed patients. in the present study, a surface ATPase (ecto-ATPase) was identified in C. neoformans yeast cells. Intact yeasts hydrolyzed adenosine-5'-triphosphate (ATP) at a rate of 29.36 +/- 3.36 nmol Pi/h x 10(8) cells. in the presence of 5 mM MgCl2, this activity was enhanced around 70 times, and an apparent K-m for Mg-ATP corresponding to 0.61 mM was determined. Inhibitors of phosphatases, mitochondrial Mg2+-ATPases, V-ATPases, Na+-ATPases or P-ATPases had no effect on the cryptococcal ATPase, but extracellular impermeant compounds reduced enzyme activity in living cells. ATP was the best substrate for the cryptococcal ecto-enzyme, but it also efficiently hydrolyzed inosine 5'-triphosphate (ITP), cytidine 5'-triphosphate (CTP), guanosine 5'-triphosphate (GTP) and uridine-5'-triphosphate (UTP). in the presence of ATP, C. neoformans became less susceptible to the antifungal action of fluconazole. Our results are indicative of the occurrence of a C. neoformans ectoATPase that may have a role in fungal physiology. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Palavra-chave Cryptococcus neoformans
Ecto-ATPase
antifungal
Idioma Inglês
Data de publicação 2005-07-01
Publicado em Fems Yeast Research. Amsterdam: Elsevier B.V., v. 5, n. 10, p. 899-907, 2005.
ISSN 1567-1356 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 899-907
Fonte http://dx.doi.org/10.1016/j.femsyr.2005.04.005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000230688800002
Endereço permanente http://repositorio.unifesp.br/handle/11600/28351

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