Reduced urinary excretion of sulfated polysaccharides in diabetic rats

Reduced urinary excretion of sulfated polysaccharides in diabetic rats

Author Lima, Cilene Rebouças de Autor UNIFESP Google Scholar
Aguiar, Jair Adriano Kopke Autor UNIFESP Google Scholar
Michelacci, Yara Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of the present study was to further understand the changes in renal filtration that occur in the early stages of diabetes mellitus. Diabetes was induced in male Wistar rats by a single injection of streptozotocin. Glycemia, body weight, 24-h urine volume and urinary excretion of creatinine, protein and glycosaminoglycans were measured 10 and 30 days after diabetes induction. All the diabetic animals used in the present study were hyperglycemic, did not gain weight, and presented proteinuria and creatinine hyperfiltration. in contrast, the glycosaminoglycan excretion decreased. Dextrain sulfates of different molecular weights (6.0 to 11.5 kDa) were administered to the diabetic rats, and to age-matched, sham-treated controls. Most of the dextran sulfate was excreted during the first 24 h, and the amounts excreted in the urine were inversely proportional to the dextran sulfate molecular weight for all groups. Nevertheless, diabetic rats excreted less and accumulated more dextran sulfate in kidney and liver, as compared to controls. These differences, which were observed only for the dextran sulfates of higher molecular weights (> 7 kDa), increased with the duration of diabetes. Our findings suggest differential renal processing mechanisms for proteins and sulfated polysaccharides, with the possible involvement of kidney cells. (c) 2004 Elsevier B.V. All rights reserved.
Keywords diabetes mellitus
renal filtration
dextran sulfate
Language English
Date 2005-06-30
Published in Biochimica Et Biophysica Acta-molecular Basis of Disease. Amsterdam: Elsevier B.V., v. 1741, n. 1-2, p. 30-41, 2005.
ISSN 0925-4439 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 30-41
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000230255600005

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