Molecular structure and transcriptional regulation by nuclear factor-kappa B of the mouse kinin B-1 receptor gene

Molecular structure and transcriptional regulation by nuclear factor-kappa B of the mouse kinin B-1 receptor gene

Autor Merino, V. F. Google Scholar
Silva, J. A. Google Scholar
Araujo, R. C. Google Scholar
Avellar, MCW Google Scholar
Bascands, J. L. Google Scholar
Schanstra, J. P. Google Scholar
Paiva, ACM Google Scholar
Bader, M. Google Scholar
Pesquero, J. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Mogi Cruzes
CHU Rangueil
Max Delbruck Ctr Mol Med
Resumo Kinins are important mediators in cardiovascular homeostasis, inflammation, and nociception. Two kinin receptors have been described, B-1 and B-2. the B-1 receptor is normally absent in healthy tissues, but is highly induced under pathological conditions. To understand the molecular mechanism of B-1 receptor up-regulation, we determined the mouse B-1 receptor gene structure, isolated and characterized the promoter region and studied its transcriptional regulation. the mouse B-1 receptor gene contains two exons (with the entire coding region located in the second exon) and a TATA-less promoter with multiple transcription start sites. A 7.7-kbp portion of the 5'-flanking region was examined for promoter activity in vascular smooth muscle cells (VSMCs). A minimal 92-bp fragment, located immediately upstream of the transcription start region, exerted basal and lipopolysaccharide (LPS)-inducible transcription activity in the sense and antisense orientation, and was thereby identified as an enhancer element. Nuclear extracts from VSMCs showed basal and LPS-inducible binding activity of nuclear factor (NF)-kappa B at this sequence. B-1 receptor transcription activation in response to LPS was abolished by cotransfection with I kappa B alpha Delta N, an NF-kappa B repressor. in summary, our results reveal the structure of the mouse B-1 receptor gene and the involvement of NF-kappa B in the inducible mouse kinin B-1 receptor expression under pathological conditions.
Palavra-chave enhancer
lipopolysaccharide (LPS)
mouse kinin B
receptor gene
multiple transcription start sites
nuclear factor-kappa B (NF-kappa B)
promoter
VSMC
Idioma Inglês
Data de publicação 2005-06-01
Publicado em Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 386, n. 6, p. 515-522, 2005.
ISSN 1431-6730 (Sherpa/Romeo, fator de impacto)
Publicador Walter de Gruyter & Co
Extensão 515-522
Fonte http://dx.doi.org/10.1515/BC.2005.061
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000230256800002
Endereço permanente http://repositorio.unifesp.br/handle/11600/28339

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