Large-volume leukapheresis for peripheral blood progenitor cell collection in low body weight pediatric patients: A single center experience

Large-volume leukapheresis for peripheral blood progenitor cell collection in low body weight pediatric patients: A single center experience

Autor Cecyn, K. Z. Google Scholar
Seber, A. Google Scholar
Ginani, V. C. Google Scholar
Goncalves, A. V. Google Scholar
Caram, E. M. Google Scholar
Oguro, T. Google Scholar
Oliveira, OMW Google Scholar
Carvalho, M. M. Google Scholar
Bordin, J. O. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
GRAAC
Resumo Peripheral blood progenitor cells (PBPC) have became the preferred source of stem cells for autologous transplantation because of easier accessibility, rapid engraftment, and lower tumor cell contamination. in pediatric patients is very important to optimize peripheral blood stem cells (PBSC) harvesting to obtain a sufficient number of cells with a reduced number of leukapheresis. in this study we prospectively analyzed data on 43 large volume leukapheresis (LVL) from 20 consecutive low body weight pediatric patients with various malignancies. Patients' mean body weight was 16.6 kg (range, 8.9-32.0 kg), and the median age was 4 years (range, 1-10 years). Instead of saline, it was used irradiated and leukoreduced red blood cell (RBC) units to prime the machine in 15 patients weighting 25 kg or less. the median number of LVL was 2 (range, 1-4) and a mean of 5.2 patient's blood volume was processed per session lasting 165 min (range, 118-239). the mean number of CD34+ cells, one day before leukapheresis was 49 mm(-3) (range, 9219). the PBPC collection yielded 24.7 x 10(8) total nucleated cells/kg (range, 6.2-74.0), 10.7 x 106 kg(-1) CD34+ cells (range, 3.6-53.7); 49.8 x 10(4) CFU-GM/kg (range, 6.4-198.1), and 65.6 x 10(4) BFU-E/kg (range, 7.6-198.1). the platelet count decreased significantly after each procedure 39.8 +/- 9.1 x 109 mm-3 (range, 18.000-76.000) (p < 0.001). in conclusion, our data show that LVL for collection of PBPC in low weight pediatric patients is a safe and efficient procedure, but it may expose the patient to the risk of thrombocytopenia. (c) 2005 Elsevier B.V. All rights reserved.
Palavra-chave large-volume leukapheresis
peripheral blood progenitor cells
pediatric patients
Idioma Inglês
Data de publicação 2005-06-01
Publicado em Transfusion and Apheresis Science. Oxford: Pergamon-Elsevier B.V., v. 32, n. 3, p. 269-274, 2005.
ISSN 1473-0502 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 269-274
Fonte http://dx.doi.org/10.1016/j.transci.2004.07.015
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000230165100007
Endereço permanente http://repositorio.unifesp.br/handle/11600/28328

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta