Interferon-gamma and tumour necrosis factor-alpha production by CD4(+)T and CD8(+)T lymphocytes in AIDS patients with tuberculosis

Interferon-gamma and tumour necrosis factor-alpha production by CD4(+)T and CD8(+)T lymphocytes in AIDS patients with tuberculosis

Autor Cunha, RMD Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Rodrigues, D. S. Google Scholar
Burattini, Marcelo Nascimento Autor UNIFESP Google Scholar
Salomão, Reinaldo Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Tuberculosis (TB) is usually more severe in HIV-infected patients, and the immune derangement found in co-infected patients may differ from that in each isolated disease. Following mitogen stimulation of peripheral blood mononuclear cells (PBMC), interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha production was evaluated in T cells by flow cytometry, and in culture supernatants by enzyme-linked immunosorbent assay (ELISA) in 33 individuals: 11 AIDS patients with tuberculosis, six asymptomatic HIV-1-infected patients, eight patients with tuberculosis and eight healthy controls. the proportion of CD4(+) T lymphocytes expressing IFN-gamma did not differ between the groups, whereas a trend towards increased proportions of TNF-alpha-expression in CD4(+) T cells was observed in the TB compared to the HIV group, while intermediate values were observed in co-infected patients. Detection of IFN-gamma and TNF-alpha in CD8(+) T lymphocytes was higher in TB than in HIV individuals. Co-infected patients presented intermediate values for IFN-gamma, while TNF-alpha detection was similar to that in HIV mono-infection. in conclusion, the proportion of T cells expressing IFN-gamma was relatively preserved in co-infected patients compared to TB patients, while the percentage of T cells expressing TNF-alpha was decreased, mainly in CD8(+) T lymphocytes. However, the marked reduction in T lymphocyte numbers in co-infected patients led to a striking reduction of both cytokines in PBMC supernatants, a finding that is consistent with the impaired response to Mycobacterium tuberculosis.
Palavra-chave AIDS
Idioma Inglês
Data de publicação 2005-06-01
Publicado em Clinical and Experimental Immunology. Oxford: Blackwell Publishing Ltd, v. 140, n. 3, p. 491-497, 2005.
ISSN 0009-9104 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing Ltd
Extensão 491-497
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000229227000011
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