Effects of intrauterine food restriction and long-term dietary supplementation with L-arginine on age-related changes in renal function and structure of rats

Effects of intrauterine food restriction and long-term dietary supplementation with L-arginine on age-related changes in renal function and structure of rats

Autor Gil, Frida Zaladek Autor UNIFESP Google Scholar
Lucas, Sandra Regina Rodrigues Autor UNIFESP Google Scholar
Gomes, Guiomar Nascimento Autor UNIFESP Google Scholar
Cavanal, Maria de Fátima Autor UNIFESP Google Scholar
Coimbra, Terezila Machado Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo We have previously demonstrated that restricting intrauterine food by 50% in 3-mo-old rats produced lower nephron numbers and early-onset hypertension, the latter being normalized by L-arginine administration. in 18-mo-old rats, such restriction increased glomerulosclerosis. in this study, we expanded our investigation, evaluating functional, morphologic, and immunohistochemical parameters in intrauterine-food-restricted 18-mo-old rats, either receiving L-arginine (RA18) or not (R18). Age-matched, non-food-restricted controls were assigned to similar groups with L-arginine (CA18) and without (C18). After weaning, L-arginine was given daily for 17 mo. No functional or morphologic changes were observed in C IS rats. the R18 rats developed early-onset hypertension, which persisted throughout the observation period, as well its significant proteinuria from 12 mo on. in RA18 rats, L-arginine decreased both blood pressure levels and proteinuria, and glomerular diameter was si,significantly smaller than in R18 rats (115.63 +/- 2.2 versus 134.8 +/- 1.0 mu m, p < 0.05). However, in RA18 rats, glomerular filtration rate remained depressed. Although L-arginine prevented glomerulosclerosis (R18 = 14%, RA18 = 4%; p < 0.05), glomerular expression of fibronectin and desmin was still greater in RA18 rats than in controls. Our data show that, although L-arginine prevented hypertension and proteinuria, glomerular injury still occurred, suggesting that intrauterine food restriction may be one of the leading causes of impaired renal function in adult life.
Idioma Inglês
Data de publicação 2005-05-01
Publicado em Pediatric Research. Baltimore: Int Pediatric Research Foundation, Inc, v. 57, n. 5, p. 724-731, 2005.
ISSN 0031-3998 (Sherpa/Romeo, fator de impacto)
Publicador Int Pediatric Research Foundation, Inc
Extensão 724-731
Fonte http://dx.doi.org/10.1203/01.PDR.0000159514.06939.7E
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000228645700020
Endereço permanente http://repositorio.unifesp.br/handle/11600/28270

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