Leptin fragments induce Fos immunoreactivity in rat hypothalamus

Leptin fragments induce Fos immunoreactivity in rat hypothalamus

Autor Oliveira, V. X. Google Scholar
Fazio, M. A. Google Scholar
Miranda, MTM Google Scholar
Silva, J. M. da Google Scholar
Bittencourt, J. C. Google Scholar
Elias, C. F. Google Scholar
Miranda, A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Leptin presents an important role in energy balance and neuroendocrine control in mammals. in an attempt to identify regions of the leptin molecule responsible for its bioactivity, we have synthesized six peptides based on the protein three-dimensional structure. Fragments were synthesized by the solid-phase methodology, purified by reverse-phase high-performance liquid chromatography (RP-HPLC), and characterized by liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS). They were injected intravenously and their ability to induce Fos immunoreactivity (Fos-ir) in rat hypothalamus was compared with that of the recombinant human leptin and saline. Fragment Ac-[Ser(117)]Lep(116-140)-NH2 (V) induced Fos-ir in hypothalamic nuclei that express leptin receptor long form. No similar ability was observed for the other five fragments. To investigate whether Fos-ir was induced in the same neuronal group activated by leptin, we proceeded with a dual-label immunohistochemistry for cocaine- and amphetamine-regulated transcript (CART), a neuropeptide related to leptin action in rat hypothalamus. We found that Ac-[Ser(117)]Lep(116-140)-NH2 (V) differentially activates CART neurons through the rostrocaudal extension of the arcuate nucleus. These results suggest that this fragment acts in the same group of neurons that mediate leptin response. This approach may offer the basis for the development of leptin-related compounds, having potential application in human or veterinary medicine. (C) 2004 Published by Elsevier B.V.
Palavra-chave synthetic peptides
obesity
CART
energy balance
Idioma Inglês
Data de publicação 2005-04-15
Publicado em Regulatory Peptides. Amsterdam: Elsevier B.V., v. 127, n. 1-3, p. 123-132, 2005.
ISSN 0167-0115 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 123-132
Fonte http://dx.doi.org/10.1016/j.regpep.2004.11.001
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000227023900015
Endereço permanente http://repositorio.unifesp.br/handle/11600/28253

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