Characterization of a RAB5 homologue in Trypanosoma cruzi

Characterization of a RAB5 homologue in Trypanosoma cruzi

Autor Araripe, M. R. Google Scholar
Ramos, F. P. Google Scholar
Silva, NLCE Google Scholar
Urmenyi, T. P. Google Scholar
Silva, R. Google Scholar
Fontes, CFL Google Scholar
Silveira, J. F. da Google Scholar
Rondinelli, E. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion. RABs show a remarkable specificity in subcellular localization. so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas' disease. RAB5 is a component of early endosomes. It has been identified in kinetoplastids such as Trypanosoma brucei and Leishmania donovani. in this work. we describe the characterization of the complete coding sequence of a RAB5 gene homologue in T. cruzi (TcRAB5, GenBank Accession No. AY730667). It is present as a single copy gene, located at chromosomal bands XIII and XIV. TcRAB5 shares the highest degrees of similarity (71%) and identity (63%) with Trypanosoma brucei rhodesiense RAB5a and contains all five characteristic RAB motifs. TcRAB5 is transcribed as a single 1.5 kb mRNA in epimastigotes. Its transcript was also detected in the other two forms of the parasite, metacyclic trypomastigotes and spheromastigotes. the recombinant TcRAB5 protein was able to bind and hydrolyze GTP. the identification of proteins involved in T. cruzi endo- and exocytic pathways may generate cellular compartment markers, an invaluable tool to better understand the vesicular transport in this parasite. (c) 2005 Elsevier Inc. All rights reserved.
Palavra-chave GTPase
Ras superfamily
RAB5
trypanosomatids
endocytic pathway
Idioma Inglês
Data de publicação 2005-04-08
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 329, n. 2, p. 638-645, 2005.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 638-645
Fonte http://dx.doi.org/10.1016/j.bbrc.2005.02.021
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000227578400031
Endereço permanente http://repositorio.unifesp.br/handle/11600/28246

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