Epstein-Barr viral load, interleukin-6 and interleukin-10 levels in post-transplant lymphoproliferative disease: A nested case-control study in a renal transplant cohort

Epstein-Barr viral load, interleukin-6 and interleukin-10 levels in post-transplant lymphoproliferative disease: A nested case-control study in a renal transplant cohort

Autor Baiocchi, Otavio Carvalho Guimarães Autor UNIFESP Google Scholar
Colleoni, Gisele Wally Braga Autor UNIFESP Google Scholar
Caballero, Otávia L. Google Scholar
Vettore, André L. Google Scholar
Bulgarelli, Adriana Google Scholar
Dalboni, Maria Aparecida Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Pestana, Jose Osmar Medina Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Ludwig Inst Canc Res
Hosp Rim & Hipertensao
Fleury Virol Div
Resumo The possible correlation among Epstein - Barr virus (EBV) load, interleukin-6 (IL-6) and interleukin-10 (IL-10) levels has become an attractive issue and can provide a useful tool for diagnosis and monitoring of patients at risk for post-transplant lymphoproliferative disease (PTLD) development. At the time of diagnosis of PTLD, 11 patients were prospectively enrolled and 55 nested controls were selected from a 1800 renal transplant cohort. Real-time polymerase chain reaction (PCR) was used to quantify EBV load in peripheral blood mononuclear cells (PBMC). Serum IL-6 and IL-10 levels were determined using an enzyme-linked immunosorbent assay ( ELISA). the median EBV load of PTLD cases was 17400 copies/10(6) PBMC, statistically different from controls ( P = 0.001). the median IL-6 level of PTLD cases was not different from controls (P=0.079). However, median IL-10 levels showed a significant difference in both groups ( P <= 0.001). the receiver-operating characteristic (ROC) curve analysis was applied to estimate the IL-10 cut-off value predictive of PTLD development. We found that 73.5 pg/ml has high sensitivity (1.00) and specificity (0.85). Also, Pearson's analysis showed a strong correlation between EBV load and serum IL-10 concentration ( P <= 0.001). This nested case - control study demonstrates that EBV load at diagnosis of PTLD correlates with IL-10 levels, and that monitoring of IL-10 can provide a less expensive and less time-consuming tool for PTLD diagnosis and close follow-up of patients at risk. Furthermore, we were able to de. ne a cut-off value of IL-10 mostly predictive of PTLD development in this cohort. Our data suggest that serial measurements prior to PTLD development must be carried out to validate our hypothesis.
Palavra-chave post-transplant lymphoproliferative disease
EBV load
IL-10
IL-6
diagnosis
Idioma Inglês
Data de publicação 2005-04-01
Publicado em Leukemia & Lymphoma. Abingdon: Taylor & Francis Ltd, v. 46, n. 4, p. 533-539, 2005.
ISSN 1042-8194 (Sherpa/Romeo, fator de impacto)
Publicador Taylor & Francis Ltd
Extensão 533-539
Fonte http://dx.doi.org/10.1080/10428190400027837
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000228805200006
Endereço permanente http://repositorio.unifesp.br/handle/11600/28231

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