Comparative analysis of genomic sequences suggests that Trypanosoma cruzi CL Brener contains two sets of non-intercalated repeats of satellite DNA that correspond to T. cruzi I and T. cruzi II types

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dc.contributor.author Elias, MCQB
dc.contributor.author Vargas, N.
dc.contributor.author Tomazi, L.
dc.contributor.author Pedroso, A.
dc.contributor.author Zingales, B.
dc.contributor.author Schenkman, S.
dc.contributor.author Briones, MRS
dc.date.accessioned 2016-01-24T12:37:45Z
dc.date.available 2016-01-24T12:37:45Z
dc.date.issued 2005-04-01
dc.identifier http://dx.doi.org/10.1016/j.molbiopara.2004.12.016
dc.identifier.citation Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 140, n. 2, p. 221-227, 2005.
dc.identifier.issn 0166-6851
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28218
dc.description.abstract Approximately 10% of the Trypanosoma cruzi. genome is formed by a satellite DNA, composed by 195-bp repeats organized in 30 +/- 10 kb clusters in some, but not all chromosomes. Here, the satellite DNA of six representative T. cruzi strains was sequenced and used for phylogenetic inference. the results show that CL Brener contains satellite repeats from T. cruzi I and T. cruzi II strains, although type II sequences are more abundant. the presence of types I and II sequences extends previous propositions that genetic exchange between the two major T. cruzi lineages have occurred in CL Brener, although our data accommodate alternative scenarios of hybridization within T. cruzi II, as proposed by others. Altogether, present data suggest a complex origin for CL Brener. Sequence analysis of satellites isolated from chromosomal bands indicates that satellite DNA sequences are not chromosome specific. Neighbor analysis of in tandem satellite DNAs containing up to five repeats shows that each cluster contains only one type of sequence. Consequently, clusters with intercalated types I and II repeats were not found. We propose that the CL Brener genome contains large pieces of satellite DNA originated mainly from chromosomes of T. cruzi II with introgression of T. cruzi I lineage. (c) 2005 Elsevier B.V. All rights reserved. en
dc.format.extent 221-227
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Molecular and Biochemical Parasitology
dc.rights Acesso restrito
dc.subject Trypanosoma cruzi en
dc.subject CL Brener en
dc.subject satellite DNA en
dc.subject phylogeny en
dc.subject hybrid genotype en
dc.title Comparative analysis of genomic sequences suggests that Trypanosoma cruzi CL Brener contains two sets of non-intercalated repeats of satellite DNA that correspond to T. cruzi I and T. cruzi II types en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1016/j.molbiopara.2004.12.016
dc.description.source Web of Science
dc.identifier.wos WOS:000228068600009



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