Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis

Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis

Autor Albuquerque, C. F. Autor UNIFESP Google Scholar
Silva, Silvia HM da Autor UNIFESP Google Scholar
Camargo, Zoilo P. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. the maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.
Idioma Inglês
Data de publicação 2005-04-01
Publicado em Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1944-1946, 2005.
ISSN 0095-1137 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 1944-1946
Fonte http://dx.doi.org/10.1128/JCM.43.4.1944-1946.2005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000228404100070
Endereço permanente http://repositorio.unifesp.br/handle/11600/28206

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