Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography

Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography

Autor Carvalho, RSH Google Scholar
Ianzer, D. A. Google Scholar
Malavolta, L. Google Scholar
Rodrigues, M. M. Google Scholar
Cilli, E. M. Google Scholar
Nakaie, C. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
UNESP
Resumo This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. the so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. in addition, the synthesized acetyl-(NANP)(3)-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence. (C) 2004 Elsevier B.V. All rights reserved.
Palavra-chave resin
peptide
polymer
ion-exchange chromatography
affinity chromatography
Idioma Inglês
Data de publicação 2005-03-25
Publicado em Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 817, n. 2, p. 231-238, 2005.
ISSN 1570-0232 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 231-238
Fonte http://dx.doi.org/10.1016/j.jchromb.2004.12.008
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000226957700014
Endereço permanente http://repositorio.unifesp.br/handle/11600/28198

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta