Ectopic development of skeletal muscle induced by subcutaneous transplant of rat satellite cells

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dc.contributor.author Fukushima, Mauricio Guendi [UNIFESP]
dc.contributor.author Furlan, Ingrid
dc.contributor.author Chiavegatti, Tiago [UNIFESP]
dc.contributor.author Kiyomoto, Beatriz Hitomi [UNIFESP]
dc.contributor.author Godinho, Rosely Oliveira [UNIFESP]
dc.date.accessioned 2016-01-24T12:37:41Z
dc.date.available 2016-01-24T12:37:41Z
dc.date.issued 2005-03-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2005000300007
dc.identifier.citation Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 3, p. 367-374, 2005.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28167
dc.description.abstract The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (106 cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodaminelabeled alpha-bungarotoxin. the development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of nAChRs (N = 2-4), resembling those from non-innervated cultured skeletal muscle fibers. After 7 days, the transplanted cells appeared as a highly vascularized tissue formed by bundles of fibers containing peripheral nuclei. the presence of X chromatin body indicated that subcutaneously developed fibers originated from female donor satellite cells. Differently from the extensor digitorum longus muscle of adult male rat (87.9 +/- 1.0 mu m; N = 213), the diameter of ectopic fibers (59.1 mu m; N = 213) did not obey a Gaussian distribution and had a higher coefficient of variation. After 7 and 14 days, the organization of the nAChR clusters was similar to that of clusters from adult innervated extensor digitorum longus muscle. These findings indicate the histocompatibility of rats from 2BAW colony and that satellite cells transplanted into the subcutaneous space of adult animals are able to develop and fuse to form differentiated skeletal muscle fibers. en
dc.format.extent 367-374
dc.language.iso eng
dc.publisher Assoc Bras Divulg Cientifica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject skeletal muscle en
dc.subject satellite cells en
dc.subject stem cells en
dc.subject transplantation en
dc.subject myogenesis en
dc.subject 2BAW rats en
dc.title Ectopic development of skeletal muscle induced by subcutaneous transplant of rat satellite cells en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIFESP, EPM, Dept Farmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliation UNIFESP, EPM, Dept Neurol & Neurocirurgia, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Farmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Neurol & Neurocirurgia, BR-04044020 São Paulo, Brazil
dc.identifier.scielo S0100-879X2005000300007
dc.identifier.doi 10.1590/S0100-879X2005000300007
dc.description.source Web of Science
dc.identifier.wos WOS:000227887100007



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