Sirolimus quantification by high-performance liquid chromatography with ultraviolet detection

Sirolimus quantification by high-performance liquid chromatography with ultraviolet detection

Autor Andrade, Maria Claudina Camargo de Autor UNIFESP Google Scholar
Di Marco, Giovana Seno Autor UNIFESP Google Scholar
Felipe, Claudia Rosso Autor UNIFESP Google Scholar
Alfieri, Fernando Google Scholar
Tedesco-Silva Junior, Hélio Autor UNIFESP Google Scholar
Pestana, José Osmar Medina Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Wyeth Ayerst Res
Resumo The need to adapt optimal conditions of sirolimus blood level monitoring in laboratories led us to optimize an high-performance liquid chromatography-ultraviolet method and compare the elution performances using the mobile phase A, 68% MeOH/2% acetonitrile (ACN)/30% H2O and mobile phase B, 30% MeOH/42% ACN/28% H2O. Samples were assayed with 1-chlorobutane, redissolved in MeOH/water and injected onto a C-18 column at 50 degrees C. the assay achieved sensitivity of 2.5-150 ng/ml (CV = 10.6%) and recovery of 92-103.6%. the intra- and interassay precisions ranged from 3.3% to 13% and from 5.9% to 15% for quality controls of 7.5, 60 and 120 ng/ml. the mobile phase A was unable to elute and recover sirolimus and internal standard in the expected retention time and concentration. Under our working conditions, the assay was precise, accurate and sensible, stressing the importance of establishing for the best working conditions according to the staff and demands of the laboratory.
Palavra-chave high-performance liquid chromatography
sirolimus
ultraviolet detection
whole blood
Idioma Inglês
Data de publicação 2005-03-01
Publicado em Transplant International. Oxford: Blackwell Publishing Ltd, v. 18, n. 3, p. 354-359, 2005.
ISSN 0934-0874 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing Ltd
Extensão 354-359
Fonte http://dx.doi.org/10.1111/j.1432-2277.2004.00066.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000227714800014
Endereço permanente http://repositorio.unifesp.br/handle/11600/28163

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta