Conformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroism

Conformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroism

Autor Andrade, S. A. de Google Scholar
Pedrosa, MFF Google Scholar
Andrade, RMG de Google Scholar
Oliva, MLV Google Scholar
van den Berge, C. W. Google Scholar
Tambourgi, D. V. Google Scholar
Instituição Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Cardiff Univ
Resumo Envenomation by arachnids of the genus Loxosceles can induce a variety of biological effects, including dermonecrosis and hemolysis. We have previously identified in L. intermedia venom two highly homologous proteins with sphingomyelinase activity, termed P1 and P2, responsible for all these pathological events, and also an inactive isoform P3. the toxins P1 and P2 displayed 85% identity with each other at the amino acid level and showed a 57% identity with SMase I, an active toxin from L. laeta venom. Circular dichroism was used to determine and compare the solution structure of the active and inactive isoforms. Effects of pH and temperature change on the CD spectra of the toxins were investigated and correlated with the biological activities. This study sheds new light on the structure-function relationship of homologous proteins with distinct biological properties and represents the first report on the structure-function relationship of Loxosceles sphingomyelinases D. (C) 2004 Elsevier Inc. All rights reserved.
Palavra-chave Loxosceles
venoms
circular dichroism
sphingomyelinase D
hemolysis
dermonecrosis
Idioma Inglês
Data de publicação 2005-02-04
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 327, n. 1, p. 117-123, 2005.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 117-123
Fonte http://dx.doi.org/10.1016/j.bbrc.2004.11.146
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000226345400019
Endereço permanente http://repositorio.unifesp.br/handle/11600/28153

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