The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil

The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil

Autor Vallochi, A. L. Google Scholar
Muccioli, C. Google Scholar
Martins, M. C. Google Scholar
Silveira, C. Google Scholar
Belfort, R. Google Scholar
Rizzo, L. V. Google Scholar
Instituição Universidade de São Paulo (USP)
Fundacao Zerbini
Brazilian Minist Sci & Technol
Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE: To determine Toxoplasma gondii genotype in scars of retinochoroiditis in humans.DESIGN: Immunopathology analysis.METHODS: DNA was isolated from retinochoroidal specimens taken from the borders of the scars from eye bank eyes as well as enucleated eyes from uveitis patients with retinochoroidal toxoplasmic scars. the SAG2 region was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism.RESULTS: of the 92 eyes Studied, 9 had toxoplasmic scars. All of them, as well as the 2 enucleated eyes, disclosed the presence of type I parasites by the gel electrophoresis analysis of the restriction fragment length polymorphism from SAG2.CONCLUSION: These results suggest that type I strain seems to be responsible for the ocular infections in the population studied.
Idioma Inglês
Data de publicação 2005-02-01
Publicado em American Journal of Ophthalmology. New York: Elsevier B.V., v. 139, n. 2, p. 350-351, 2005.
ISSN 0002-9394 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 350-351
Fonte http://dx.doi.org/10.1016/j.ajo.2004.07.040
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000227454200023
Endereço permanente http://repositorio.unifesp.br/handle/11600/28132

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